乌灵菌粉的镇静作用及其机理研究

 目的:研究真菌中药乌灵菌粉对小鼠的镇静安眼作用及其机制?方法:以常规的药理试验方法检测本药在戊巴比妥钠协同下对小鼠睡眠的影响;以同位素掺入法研究作用机理?结果:乌灵菌粉在戊巴比妥钠协同下有明显的促睡眠作用,并使自主活动减少?同时服药小鼠脑内谷氨酸(Glu)和γ-氨基丁酸(GABA)的含量及GABA受体的结合活性均高于对照,药物还能提高谷氨酸脱羧酶(GAD)的活性?结论:乌灵菌粉可能是通过促使Glu,GABA进入脑内,提高GAD活性及激活GABA受体而实现其镇静作用?

Studies on the sedative and sleeping effects of Wuling mycelia and its pharmacological mechanism

OBJECTIVE: To reveal the sedative and sleeping effcets of Wuling mycelia (a Chinese medicine) and the mechanism involved.METHODS: By using the routine pharmacological methods,the synergistically sedative and sleeping effects of Wuling mycelia and nembutal sodium were detected in mice,while the mechanism involved was investigated by isotope incorporation assay. RESULTS: Powdered Wuling mycelia in combination with nembutal sodium could promote sleep and decrease autonomic locomotions of mice.After administration of Wuling mycelia,the contents of glutamic acid (Glu) and aminobutyric acid (GABA) and the binding affinity of GABA receptors in the brain were found to be augmented as compared with those of controls.Further,the activity of glutamate decarboxylase (GAD) could also be elevated. CONCLUSION: The data suggested that Wuling mycelia possesses the ability to facilitate the entry of Glu and GABA into the brain,to activate the receptors of GABA and thus to exert its sedative and sleeppromoting properti