台湾金线莲和菌根真菌互作过程中抗旱相关蛋白质组差异

 目的 研究干旱条件下接种菌根真菌的植物与未接菌植物的蛋白质组差异,探索菌根真菌提高植物抗旱能力可能的机制。方法 以未接菌植物作对照,采用双向电泳法首次从真菌、植物和环境3因素上研究在干旱逆境中接种丝核菌属的台湾金线莲与未接菌对照的蛋白质组差异。结果 差异表达蛋白中涉及光合作用的有8个,其中7个与暗反应中CO₂的固定相关,并且植物启动了干旱条件下的C₄途径以充分利用CO₂;涉及糖、脂代谢的有5个蛋白,蛋白质合成相关蛋白有3个,抗性相关蛋白2个。结论 干旱条件下接菌台湾金线莲的光合作用的暗反应尤其是C₄途径加强,增加了在气孔关闭情况下固定和利用CO₂的能力,糖、脂代谢和蛋白合成及抵抗病虫害的能力均强于未接菌对照;丝核菌属菌株能够帮助台湾金线莲增加抵御干旱的能力。

Anti-Drought Differential Proteome on Interaction between Anoectochilus formosanus and Mycorrhizal Fungi

Objective To study the proteomic difference between mycorrhizal fungi infected and uninfected plants and then to explore the possible anti-drought mechanism caused by mycorrhizal fungi. METHODS The differential proteome of Anoectochilus formosanus infected by Rhizoctonia sp. growing in drought was investigated by means of 2D gel electrophoresis. RESULTS Eight proteins were involved in photosynthesis,among them 7 proteins were correlated with CO₂ fixation of darkreaction. Meanwhile,C₄ pathway suited in drought condition was mobilized by the plants to utilize CO₂ thoroughly. Five proteins were involved in glycometabolism and lipometabolism,and 3 in protein synthesis. CONCLUSION Increased darkreaction of photosynthesis,especially the C₄ pathway in infected fungi can enhance the capability of CO₂ fixation and utilization when the stoma is closed. The glycometabolism,lipometabolism,protein synthesis,and resistance to pests of the infected plants are also enhanced in drought condition. Fungus of Rhizoctonia sp. can help plants improve their capability to resist drought.