菟丝子拟雌激素活性的谱效相关性研究

 目的 探求不同产地菟丝子指纹图谱的雌激素样作用,为建立“指纹图谱与药效关系”的中药质量标准控制提供依据。方法 HPLC建立菟丝子不同产地的指纹图谱,子宫增重法和MTT实验筛选出菟丝子雌激素样作用的有效成分,采用双变量相关分析谱效关系。结果 建立了菟丝子药材的指纹图谱,确立26个色谱峰为共有峰。与空白组和阳性对照组比较,20号产地对小鼠子宫增重和MCF-7细胞增殖作用显著。其雌激素作用为多种化学成分共同作用的结果。结论 菟丝子不同产地的指纹图谱与其雌激素作用有一定的对应关系。

Spectrum-Effect Correlation of Mimic Estrogenic Activity of Seeds of Cuscuta chinensis

Objective To explore the relationship between the estrogen-like effect of seeds of Cuscuta chinensis and the fingerprints of the seeds of Cuscuta chinensis from different habitats,thus to provide a basis for the establishment of Chinese medicine quality standards based on the relationship between the fingerprinting and pharmacodynamics. METHODS The fingerprints of seeds of Cuscuta chinensis samples from different habitats were established by HPLC,and the active ingredients of seeds of Cuscuta chinensis with estrogen-like effect were screened by uterotrophic method and MTT assay,and the spectrum-activity relationship was analyzed by bivariate correlation analysis. RESULTS Twenty-six peaks of seeds of Cuscuta chinensis Lam. were identified as common peaks by HPLC. It was shown by uterotrophic and MTT assay that the seeds of Cuscuta chinensis from No. 20 habitat could significantly promote the proliferation of MCF-7 cells and the increase of uterus weight compared with the blank control group and the positive control group. It was shown that the estrogenic effect of seeds of Cuscuta chinensis was the combined effect of a variety of chemical constituents. CONCLUSION There exsists correlation between the fingerprints of the seeds of Cuscuta chinensis from different habitats and their estrogenic activity.