银杏叶提取物(EGb761)拮抗阿霉素引起的心脏毒性不影响其抗肿瘤活性

 目的：研究银杏叶提取物（EGb761）对阿霉素心脏毒性以及抗肿瘤活性的影响。方法：采用阿霉素体外损伤大鼠心脏线粒体和体内小鼠心脏毒性两种模型，应用生化方法和电镜观察药物作用。结果：阿霉素体外引起大鼠心脏线粒体丙二醛生成、腺苷三磷酸酶活性丧失、膜流动性降低、膜线粒体肿胀、溶解等，EGb761对上述毒性损伤均有明显的保护作用。体内实验表明，EGb761能剂量依赖性地抑制阿霉素引起的小鼠心肌脂质过氧化和血清肌酸磷酸激酶活性升高。荷瘤鼠存活期实验表明，EGb761对阿霉素抗肿瘤活性无明显影响。结论：EGb761拮抗阿霉素引起的心脏毒性但不影响其抗肿瘤活性，这对临床应用EGb761以减轻阿霉素对心脏毒性的可能性提供了实验依据。

Protection of Ginkgo biloba extract (EGb761) against doxorubicin-induced cardiotoxicity without interfering with its antitumor activity

OJECTIVE：To explore whether EGb761 could protect against doxorubicin-induced cardiotoxicity, and whether it affects on doxorubicin antitumor activity. METHOD： Doxorubicin-induced rat heart mitochondria damage in vitro and doxorubicin-induced cardiotoxicity in mice were used. The cardiotoxicity were evaluated via spectrophotography, electromicrography and other methods.RESULTS： Doxorubicin markedly induced malondialdehyde formation, adenosine 5′-triphosphatase activity loss, membrane rigidification, swelling, disintegration and lysis of rat heart mitochondria in vitro, all the above pathological and biochemical damages were reduced significantly by EGb761. Cardiotoxicity of doxorubicin in mice as measured by increases of heart Malondialdehyde level and serum creatine phosphokinase activity was significantly alleviated by EGb761. Moreover. The antitumor activity of doxorubicin, as assayed by prolonged life span of mice bearing with H22 hepatoma, was not reduced by EGb761 treatment. CONCLUSION：Ginkgo biloba extract may protect heart against doxorubicin-induced cardiotoxicity without interfering with its antitumor activity in mice.