硫酸铵梯度法制备米托蒽醌长循环脂质体

目的：利用跨膜硫酸铵梯度法制备米托蒽醌长循环脂质体并对其质量进行评价。方法：采用乙醇注入合并硫酸铵梯度法制备米托蒽醌长循环脂质体。用两亲性聚乙二醇-二硬脂酰磷脂乙醇胺（PEG-DSPE）修饰脂质体膜，并用Sephadex G50葡聚糖凝胶柱分离，紫外分光光度法测定米托蒽醌脂质体的包封率，以激光散射粒径分析仪和电子显微镜测定米托蒽醌脂质体的粒径。结果：长循环脂质体平均粒径为60nm，药物包封率为93.65％。脂质体外观圆整而均匀。结论：采用乙醇注入合并硫酸铵梯度法可制得包封率高、粒径小的脂质体，而且方法简便易行。

Studies on preparation of long circulating mitoxantrone liposomes with transmembrane ammonium sulfate gradients

OBJECTIVE To investigate the preparation of the lo ng circulating mitoxantrone liposomes with ammonium sulphate gradients method an d to evaluate the quality of the liposomes.METHODS The long circulating mitoxantrone liposomes were prepared by ammonium sulphate gradients method. The amphipathic polyethylene glycol distearoyl phosphatidylethanolamine (PEG DSPE) was added to modify the membrane of the liposomes. Ultraviolet spec trophotometry was utilized to determine the drug entrapping efficiency of liposo mal mitoxantrone. Electroscopy and laser particle analyzer were applied to deter mine the size of mitoxantrone miposomes.RESULTS The mean dia meter of the long circulating mitoxantrone liposomes was 60 nm, with the entrapp ing efficiency of 93.65%.The liposomes had perfect shape.CONCLUSION The liposomes with high entrapping efficiency and small particle size co uld be prepared by ammonium sulphate gradients method, which is applied easily.