海洋来源真菌Penicillium sacculum次级代谢产物的研究

 目的 研究海洋来源真菌Penicillium sacculum的次级代谢产物。方法 采用多种色谱方法进行分离纯化，通过理化性质和波谱数据鉴定化合物的结构；采用台盼蓝排斥法和MTT法进行抗肿瘤活性测试。结果 从发酵液和菌丝体中共分离得到10个化合物，结构鉴定为(R)-1， 3-二氢-6-甲氧基-3-甲基异苯并呋喃-4， 5-二醇(1)、灰黄霉素(2)、1， 6-二羟基-3-甲氧基-8-甲基酮(3)、1-羟基-3-甲基酮(4)、1， 3， 6-三羟基-8-甲基酮(5)、1， 8-二羟基-3-甲基蒽醌(6)、1， 3， 5-三羟基-7-甲基蒽醌(7)、6-羟基芦荟大黄素(8)、腺嘌呤(9)、胸腺嘧啶(10)；化合物1和3对人急性髓细胞性白血病细胞HL-60分别具有较强和中等强度的抑制活性。结论 化合物1和4均为首次从该属真菌中分离得到；首次对化合物1的¹H-NMR和¹³C-NMR谱信号进行了全归属；化合物1和3具有抗肿瘤活性。

Study on the Secondary Metabolites of Marine-Derived Fungus Penicillium sacculum

OBJECTIVE To study the secondary metabolites of marine-derived fungus Penicillium sacculum.METHODS The compounds were separated by several column chromatographic techniques,and their structures were elucidated by means of physicochemical properties and spectral data.Trypan blue exclusion assay and MTT method were used to test the anti-tumor activities.RESULTS Ten compounds were isolated from the fermentation broth and mycelium.Their structures were identified as(R)-1,3-dihydro-6-methoxy-3-methylisobenzofuran-4,5-diol(curvulol)(1),griseofulvin(2),1,6-dihydroxy-3-methoxy-8-methylxanthone(3),1-hydroxy-3-methylxanthone(4),1,3,6-trihydroxy-8-methylxanthone(5),1,8-dihydroxy-3-methylanthraquinon(6),1,3,5-trihydroxy-7-methylanthraquinone(7),citreorosein(8),adenine(9) and thymine(10).Among them,compounds 1 and 3 showed respectively potent and moderate growth inhibitory activities against human leukemia HL-60 cells.CONCLUSION Compounds 1 and 4 were isolated for the first time from the genus of marine-derived fungus.The 1H-NMR and 13C-NMR signals of compound 1 were assigned for the first time.Compounds 1 and 3 have anti-tumor activities.