苦参碱对皮肤角质形成细胞IL-17R、p-p38 MAPK和炎症因子的影响

 目的 考察苦参碱（matrine，Mat）对皮肤角质形成细胞（keratinocytes， KC）系HaCaT细胞白细胞介素-17受体（IL-17R）、p-p38 MAPK表达的影响，及其对HaCaT细胞分泌白细胞介素（IL）-6、IL-8、MIP-3α等炎症因子的影响。方法 采用RT-PCR方法检测Mat对皮肤KCIL-17R mRNA表达的影响；采用流式细胞术检测Mat对HaCaT细胞IL-17R蛋白含量的影响；采用Western blot方法检测Mat对HaCaT细胞表达p-p38 MAPK的影响；ELISA法检测Mat对HaCaT细胞分泌炎症因子IL-6、IL-8及MIP-3α的影响。结果 Mat可降低HaCaT细胞表达IL-17R和p-p38 MAPK，以及降低HaCaT细胞IL-6、IL-8、MIP-3α等炎症因子的分泌。结论 Mat可通过抑制IL-17R的表达，阻止p38 MAPK的磷酸化过程而具有抗炎作用。

Effects of Matrine on IL-17R,p-p38 MAPK and Inflammatory Factors in Cultured Keratinocytes

OBJECTIVE To investigate the effect of matrine on the expression of IL-17R， p-p38 MAPK in skin keratinocyte cell line HaCaT cells， and on the level of IL-6， IL-8， MIP-3α secreted by HaCaT cells. METHODS RT-PCR method was applied to analyze the expression of IL-17R mRNA in HaCaT cells treated with matrine. Flow cytometry was used to analyze the IL-17R protein content. The expression of p-p38 MAPK was determined by Western blot method. The levels of inflammatory factors IL-6， IL-8 and MIP-3α were analyzed by ELISA. RESULTS Matrine decreased the expression of IL-17R and p-p38 MAPK in HaCaT cells， and the level of IL-6， IL-8 and MIP-3α secreted by HaCaT cells. CONCLUSION Matrine can inhibit the expression of IL-17R to prevent the phosphorylation of p38 MAPK， and inhibit the expression of IL-6， IL-8， MIP-3α， and so matrine has anti-inflammatory effect.