新型阳离子基因传递载体聚乙烯亚胺-醋酸酐的细胞转染研究

 目的 研究非病毒基因载体聚乙烯亚胺（PEI）-醋酸酐（ACA）共聚物的组成对体外介导基因传递的影响，评价其作为基因载体的可行性。方法 通过粒径分析观察PEI-ACA与质粒DNA自组装形成的颗粒形态及粒径，预测其进入细胞的可能性。以MTT法检测PEI-ACA和PEI的细胞毒性。以PEI-ACA、PEI为载体，在不同的N/P比值的条件下，将绿色荧光蛋白基因pEGFP-C3导入真核细胞株A549细胞，以荧光显微镜及荧光分光光度计系统检测其转染效率。结果 PEI-ACA转染效率高于PEI，同时PEI-ACA的细胞毒性显著低于PEI。结论 新型阳离子多聚物PEI-ACA提高PEI介导的基因转染效率同时降低其细胞毒性，有望成为基因转染的有效载体。

Transfection with a Novel Cationic Gene Carrier： Polyethylenimine-Acetic Anhydride

OBJECTIVE To study the influence of copolymer polyethylenimine(PEI)-acetic anhydride(ACA) composition on gene delivery in vitro and observe its potential as a gene carrier．METHODS Size measurement and AFM were used to show the modality of the PEI-ACA/DNA complexes．The cytotoxicity of PEI-ACA and PEI was evaluated by mononuclear cell direct cytotoxicity assay (MTT). In different conditions，using PEI-ACA as the vector，A549 cells were transfected with fluorescent protein (pEGFP-C3) gene and 1ueiferase gene．Expression efficiency of the transduced gene was monitored by fluorescence microscopy and fluorescence assay system．RESULTS PEI-ACA had higher transfection efficiency with lower cytotoxicity than PEI. CONCLUSION PEI-ACA copolymer reduces the cytotoxicity of PEI，improves the transfection efficiency，and offers greater biocompatibility than PEI．It shows considerable potential as an efficient non-viral carrier for gene delivery．