铁线蕨水煎液治疗泌尿系统感染实验研究

 目的 探讨铁线蕨体内外抑菌、利尿等作用。方法 采用二倍稀释法观察铁线蕨对几种常见致病菌的体外抑制作用;建立急性系统性白念菌株感染小鼠模型,以小鼠存活率,脾脏活菌数和肾脏病理变化为指标观察该植物对感染小鼠的保护作用;建立水钠潴留的大鼠模型,观察其对大鼠尿量的影响。结果 铁线蕨的水煎液对几种供试菌均具有抑菌活性;可显著延长急性系统性感染白念菌株小鼠的生存时间,减少脾脏中的菌落数,改善肾脏的病理变化,对感染小鼠具有一定的保护作用;对水钠潴留的模型大鼠尿量具有双向调节作用,低剂量增加尿量,缩短排尿潜伏期,高剂量减少尿量。结论 铁线蕨在一定的剂量范围内具有良好的体内外抑菌活性和利尿作用,为其治疗泌尿系统感染性疾病提供了实验依据。

Effect of Water Extracts of Adiantum capillus-veneris L.on Urinary Tract Infections

OBJECTIVE To investigate the biological properties of A. capillusveneris L. including in vitro and in vivo antimicrobial efficacy and diuretic efficacy. METHODS Double-fold dilution method was employed to observe the baceteriostatic action of the drug in vitro. Systemic Candida albicans infection model were established in mice to evaluate the protective effects of A. capillusveneris L. in terms of survival rate, splenic bacteriuria counts and renal pathological characteristics. The urinary effect of A. capillusveneris L. was studied in the rats loading with normal saline. RESULTS The water extracts of A. capillusveneris L. showed certain inbibitional effect on all tested bacterial species in the experiments. It prolonged the life of Systemic Candida albicans infection model mice, decreased the colony-forming units (CFU) of Candida albicans in the spleen and ameliorated the renal pathological characteristics. Moreover, it showed doubled effects on diuresis actions. The low dosage increased the urinary output, shorten the latent period of emiction, and the high dosage decreased the urinary output. The results showed that this drug effectively protected the infectious mice. CONCLUSION The water extracts of A. capillusveneris L. had in vitro and in vivo antimicrobial efficacy and diuretic efficacy, which provided strong pharmacologic proofs for its further treatment of urinary tract infections (UTI).