高效毛细管电泳法测定葛根及其制剂脑得生片中葛根素的含量

 目的 测定葛根(野葛、粉葛)及其制剂脑得生片中葛根素的含量。方法 采用高效毛细管电泳法，双氯灭痛为内标物。测定条件为：运行缓冲液：30 mmol·L^-1硼砂缓冲溶液(pH 9.37)；操作电压30 kV，紫外检测波长254 nm。结果 葛根素的线性范围为10～100 μg·ml^-1，葛根的平均回收率为96.77%( RSD 2.65%，n=3)；脑得生片平均回收率为：100.64%( RSD 0.28%，n=3)。结论 该方法简便、快捷，具有良好的精密度、回收率和线性关系，结果满意。

Determination of puerarin in radix puerariae and naodesheng tablets by HPCE

OBJECTIVE To determine the contents of puerarin in radix puerariae and Naodesheng tablets. METHODS A HPCE method was used.Diclophenac sodium ws used as the internal standard.HPCE conditions were:buffer＝30 mmol·L^-1(borate,pH=9.37);applied voltage 30 kV(positive to negative),UV detector λ=254 nm. RESULT The standard curve was linear over the range of 10～100 μg·ml^-1with the correlation coefficient 0.9998.The average recovery was 96.77%(RSD=2.65%,n=3) for radix pueraiae and 100.64% (RSD=0.28%,n=3) for Naodesheng tablets. CONCLUSION The method is simple and fast for analysis of Puerarin.