枳椇子提取物对D-半乳糖致亚急性衰老小鼠氧化损伤的保护作用

 目的观察枳椇子提取物(Semen hoveniae extract,SHE)对D-半乳糖诱导的亚急性衰老小鼠氧化损伤的保护作用,并探讨其保护作用的可能机制。方法NIH小鼠随机分为6组:空白对照组,衰老模型组,维生素E组(200mg·kg^-1·d^-1),SHE低、中、高剂量组(50,100,200mg·kg^-1·d^-1),小鼠颈背部皮下注射D-半乳糖(100mg·kg^-1·d^-1)造成小鼠亚急性衰老模型,同时按分组灌胃给予相应药物,每天一次,连续给药45d后进行实验,以小鼠水迷宫测定衰老小鼠的学习记忆能力;比色法测定小鼠肝脏及脑组织中超氧化合物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量,蛋白含量测定用考马斯亮蓝法。结果水迷宫实验中,与模型组小鼠相比,各剂量给药组小鼠到达平台的潜伏期明显缩短,进入盲端的错误次数显著减少;肝、脑组织匀浆测定结果显示:各给药组SOD、GSH-Px活力显著增加,MDA含量明显减少。结论不同剂量的SHE均能改善D-半乳糖致亚急性衰老小鼠的学习记忆能力,具有延缓衰老的作用,此作用可能部分是通过增加体内抗氧化酶的活性,减少过氧化脂质的生成,提高机体抗氧化能力而导致的。

Protective Effect of Semen hoveniae Extract on Oxidative Injury in Subacute Senile Mice Induced by D-galactose

OBJECTIVE To study the protective effect and investigate the underlying mechanisms of Semen hoveniae extract(SHE) on oxidative injury in subacute senile mice induced by D-galactose.METHODS NIH mice were randomly divided into 6 groups:control group,aging model group,vitamin E(positive control) group(200 mg·kg^-1·d^-1),and SHE groups(50,100,200 mg·kg^-1·d^-1).The subacute aging model of mice were induced by D-galactose(100 mg·kg^-1·d^-1) following s.c.administration,at the same time,SHE or vitamin E were given ig for 45 d.The learning and memory ability of mice were tested by water maze,the activities of superoxide dismutase(SOD),glutathione(GSH-Px) and the contents of malondialdehyde(MDA) in the brain and liver were measured by colorimetric method.RESULTS Compared with the model group,the escape latency was shortened and the times of entry errors were decreased significantly in the mice of SHE treatment groups and vitamin E group.The extract obviously strengthened the activities of SOD and GSH-Px in liver and brain tissue,reduced the content of MDA.CONCLUSION SHE could improve the learning and memory ability of senile mice,the extract has significant protective effect on oxidative injury and the mechanisms probably related to its improvement of antioxidases and reduction of lipid peroxidation.