HPLC测定鼻炎灵喷剂中4种组分的含量

 目的：测定鼻炎灵喷剂中盐酸麻黄碱、呋喃西林、地塞米松磷酸钠、盐酸苯海拉明的含量。方法：高效液相色谱法，国产C₁₈柱；检测波长258 nm；流动相A液为0.025 mol/L磷酸二氢钾溶液-甲醇-乙腈(77∶3∶20)，B液为甲醇；梯度洗脱。结果：盐酸麻黄碱0.60～3.60 mg/ml，呋喃西林0.012～0.07 mg/ml，地塞米松磷酸钠0.018～0.11mg/ml，盐酸苯海拉明0.10～0.61 mg/ml范围内，峰面积与其浓度呈良好线性关系；平均回收率依次为99.95%，RSD=0.78%；101.7%，RSD=0.50%；101.9%，RSD=0.57%；101.4%，RSD=0.58%。结论：方法简便、快速、准确，可作为样品的检测方法。

Determination of ephedrine,furacilin,dexamethasone and diphenhydramine in Biyanling nasal spray by HPLC

OBJECTIVE:To separate and determine four constituents including ephedrine hydrochlorid(EPD),furacilin,dexamethasone and diphenhydramine hydrochloride(DPH) in Biyanling nasal spray.METHODS:A HPLC method was used.Chromatographic conditions included C₁₈ column(4.6 mm×200 mm;4 μm);mobile phase,solution A:0.025 mol/L KH₂PO₄-methanol-acetonitrile(77∶3∶20),solution B:methanol,gradient elution;detection wavelength 258 nm.RESULTS:The calibration curves were linear in the range of 0.60～3.60 mg/ml for EPD;0.012～0.07 mg/ml for furacilin;0.018～0.11 mg/ml for dexamethasone and 0.10～0.61 mg/ml for DPH.The average recoveries of EDP,furacilin,dexamethasone and DPH were 99.95%(RSD=0.78%),101.7%(RSD=0.50%),101.9%(RSD=0.57%),and 101.4%(RSD=0.58%) respectively.CONCLUSION:This method was found to be simple,quick and accurate for simultaneous determination of the four constituents in Biyanling nasal spray.