大孔树脂分离纯化三叶青总黄酮的工艺研究

 目的 筛选出分离纯化三叶青总黄酮的较佳树脂,并对影响分离纯化的主要因素进行研究,得到优化的分离条件。方法 利用分光光度法测定三叶青总黄酮的含量;选择了10种国产大孔树脂,以三叶青总黄酮的吸附量和解吸率为指标,筛选出较优的树脂,并对其静态、动态吸附与解吸性能进行研究,进而优化工艺条件。 结果 HPD826树脂具有较好的吸附和解吸性能,且吸附平衡数据符合Freundlich 吸附等温模型,其较优的工艺如下：上样流速以2 BV·h-1为佳,上样后先用2 BV水洗脱,然后用3 BV、体积分数70%的乙醇以2 BV·h-1的流速洗脱。经HPD826大孔树脂一次纯化后的产物中,三叶青总黄酮含量提高了2.44倍。结论 HPD826大孔树脂能较好地用于分离纯化三叶青总黄酮。

Separation and Purification Process of Total Flavones from Radix tetrastigmae with Macroporous Resins

OBJECTIVE To screen the suitable resin for separation and purification of total flavones from Radix tetrastigmae, and to establish the optimum parameters of separation and purification process. METHODS The adsorption capacities and desorption ratios of ten selected macroporous resins were evaluated with UV method. Suitable resins were chosen to study the performance of static-dynamic adsorption and desorption, and to optimize the process parameters. RESULTS HPD826 resin showed advantageous adsorption and desorption properties with thermodynamic parameter according with the Freundlich equation. The optimal conditions were determined as follows for adsorption, the flow speed was 2 BV·h-1; for desorption, wash the adsorbate-laden column first with 2 BV water, then with 3 BV 70% ethanol-water. Under this condition, the content of total flavones increased by 2.44-fold. CONCLUSION It is feasible to separate and purify Radix tetrastigmae total flavones with HPD826 resin.