四倍体菘蓝毛状根的培养及其抗内毒素成分分析

 目的 从菘蓝的同源四倍体植物诱导出毛状根并探讨用毛状根培养来大规模生产有用的药用植物次生代谢物的可能性。方法 利用发根农杆菌A4菌株对四倍体菘蓝进行感染，采用高效毛细管电泳法同时测定诱导出的毛状根中具有抗内毒素作用的五种有机酸［邻氨基苯甲酸、水杨酸、丁香酸、苯甲酸、3-(2-苯甲酸)-4(3H)-喹唑酮］的含量。结果 A4菌株从四倍体菘蓝诱导出毛状根，且毛状根含有原植物中具有抗内毒素作用的五种有机酸。结论 发根农杆菌能诱导四倍体植物产生毛状根，我们有可能利用毛状根培养来生产有价值的植物次生代谢物。

Studies on culture of hairy roots of autotetraploid Isatis indigotoca and analysis of its antiendotoxic active components

OBJECTIVE To induce the hairy roots autotetraploid Isatis indigotoca Fort.and discuss the probability to produce useful botanic secondary metabolites from hairy root culture on a large scale. METHODS The cotyledon leaf explants of autotetraploid Isatis indigotoca Fort.were infected by Agrobacterium rhizogenes strain A4,and five antiendotoxic active organic acids ［Anthranilic acid,salicylic acid,syringic acid,benzoic acid and 3-(2-carboxyphenyl)-4(3H)-quinazolinone］ were determined simultaneously by high performance capillary electrophoresis. RESULTS The hairy roots of autotetraploid Isatis indigotica Fort.could be indced by Agrobacterium rhizogenes strain A4 and the hairy roots contained same five antiendotoxic active components as that in the primary plant. CONCLUSION Agrobacterium rhizogenes could induce hairy roots from polyploid plants and the hairy roots might be utilized to produce valuable botanic secondary metabolites.