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Antibacterial activity of honey in north-west Pakistan against select human pathogens
Authors:Ali Talha Khalil  Imran Khan  Kafeel Ahmad  Yusra Ali Khan  Jangrez Khan  Zabta Khan Shinwari
Institution:1. Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan;2. Centre of Biotechnology and Microbiology, University of Peshawar, Peshawar 25000, Pakistan;3. Department of Biochemistry, Quaid-i-Azam University, Islamabad 45320, Pakistan
Abstract:

Objective

To investigate the antimicrobial activity of commercially available honey and raw honey samples in Khyber Pakhtunkhwa, Pakistan, against pathogenic bacterial strains.

Methods

Well diffusion assays were performed to screen pure and diluted honey samples for antibacterial activity against six Gram-negative and six Gram-positive bacterial strains. Zones of inhibition were measured and compared with 10 mg Gentamycin.

RESULTS

When honey samples were diluted to 20%-70%, the honey samples showed no activity to mild antibacterial activity. The highest antibacterial activity was recorded when 90% and pure undiluted honey samples were tested and compared with a control Gentamycin disc (10 mg).

Conclusion

Commercially processed honey and raw honey samples from north-west of Pakistan possess good antimicrobial potential.
Keywords:Anti-bacterial agents  Disk diffusion antimicrobial tests  Honey
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