| Gas chromatography-mass spectrometry analysis of essential oils from five parts of Chaihu （Radix Bupleuri Chinensis） |
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| 作者姓名： | Jie Meng ;Xingfu Chen ;Wenyu Yang ;Jiuhua Song ;Yu Zhang ;Zhifei Li ;Xingwang Yang ;Zhanguo Yang |
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| 作者单位： | [1]Department of Pharmaceutical Botany, College of Agronomy, Sichuan Agricultural University; [2]Key Laboratory of Crop Physiology, Ecology and Cultivation in Southwest, Ministry of Agriculture, Chengdu 611130, China; [3]Production Division, Sich-uan Depeiyuan Traditional Chinese Medicine TechnologyDevelopment Co., Ltd., Mianyang 621000, China |
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| 基金项目： | Breeding Research Project of Chinese Medi- cal Herbs in Sichuan Province：Study on the breeding of new Chaihu variety and cultivation and the related techniques （No. 2011NZ0098-12-11）, China Spark Program in 2010 （No. 2010GA810056） |
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| 摘 要： | OBJECTIVE： To analyze the essential oils from flowers, leaves, stems, roots, and fruits of Chaihu（Radix Bupleuri Chinensis）.METHODS： We extracted essential oils from different parts of Chaihu（Radix Bupleuri Chinensis） using a steam distillation method. The essential oils were analyzed by gas chromatography-mass spectrometry（GC-MS）. Data were collected in full scan mode（m/z 60-600）. Volatile components were identified based on their retention indices and by comparing their mass spectra with those in the National Institute of Standards and Technology 2005 database assisted by tandem mass spectrometry information. The relative content of each constituent wasdetermined by area normalization.RESULTS： We identified 111 components, of which12 were common to all 5 parts, 30 were found only in roots, 14 were found only in flowers, 6 were found only in leaves, 4 were found only in stems,and 17 were found only in fruits.CONCLUSION： Our results show that the stems,flowers, leaves, and fruits of Chaihu（Radix Bupleuri Chinensis）contain a high concentration of essential oils, and that the exact composition of the essential oils differs among the plant parts. To develop new medicines and make full use of the Chaihu（Radix Bupleuri Chinensis）resource, it is important to characterize the essential oils from different parts of the plant. In future research, it will be important to determine the pharmacological effects of the various components and the essential oil mixtures.
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| 关 键 词： | 气相色谱 质谱图 柴胡 精油 羊草 光谱分析 水蒸气蒸馏法 美国国家标准 |
Gas chromatography-mass spectrometry analysis of essential oils from five parts of Chaihu （Radix Bupleuri Chinensis） |
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| Jie Meng,;Xingfu Chen,;Wenyu Yang,;Jiuhua Song,;Yu Zhang,;Zhifei Li,;Xingwang Yang,;Zhanguo Yang.Gas chromatography-mass spectrometry analysis of essential oils from five parts of Chaihu （Radix Bupleuri Chinensis）[J].Journal of traditional Chinese medicine,2014(6):741-748. |
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| Abstract: | Gas chromatography-mass spectrome-try Oils, volatile Chaihu （Radix Bupleuri Chinensis） |
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| Keywords: | Gas chromatography-mass spectrome-try Oils volatile Chaihu （Radix Bupleuri Chinensis） |
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