健脾解毒方对奥沙利铂抑制人结肠癌裸鼠原位移植瘤生长的增效作用

目的:观察健脾解毒方对奥沙利铂抑制人结肠癌裸鼠原位移植瘤生长的增效作用,并初步探讨其作用机理。方法:建立人结肠癌裸鼠原位移植瘤模型,随机分为4组:对照组、奥沙利铂组、健脾解毒方组、健脾解毒方联合奥沙利铂组(联合用药组),观察各组抑瘤率、裸鼠体重及生存期;采用MTT法检测治疗后移植瘤肿瘤细胞耐药性的变化。结果:(1)联合用药组的抑瘤率为53.6%,是单用奥沙利铂组的4.25倍。(2)治疗后,健脾解毒方组和联合用药组体重较对照组增加(P0.05)。(3)健脾解毒方组和联合用药组中位生存期长于对照组和奥沙利铂组(P0.05)。(4)奥沙利铂组对L-OHP、5-Fu、HCPT和THP的耐药指数分别为7.59、4.28、5.78和4.50;联合用药组对L-OHP、5-Fu、HCPT和THP的逆转指数分别为6.57、2.61、4.97和3.10。结论:健脾解毒方对奥沙利铂抑制人结肠癌裸鼠原位移植瘤生长有增效作用,其增效作用可能与健脾解毒方部分逆转移植瘤对奥沙利铂的耐药性有关。

The Synergistic Effect of Jianpi Jiedu Fang on Orthotopic Transplantation Nude Mouse Mode of Human Colon Carcinoma Treated by the Oxaliplatin

Objective:To evaluate the effect of Jianpi Jiedu Fang(JPJDF)on the inhibition of oxaliplatin on the orthotopic transplantation colon cancer.Methods:The orthotopic transplantation nude mice mode of human colon carcinoma were established and randomized were randomly dividied into four groups:control group,L-OHP group,JPJDF group,L-OHP combined with JPJDF groups,and they were treated with different drugs respectively.We observed the tumor inhibition rates,the weight and survival time,After the primary culture,...