补肺益肾方对幼龄哮喘豚鼠模型气道重塑中嗜酸粒细胞凋亡的影响

目的:探讨补肺益肾方对幼龄哮喘豚鼠气道重塑和支气管肺泡灌洗液(BALF)中白介素-5(IL-5)水平和嗜酸粒细胞(EOS)凋亡的影响。方法:24只健康幼龄豚鼠随机分为正常组、模型组、中药组。以卵蛋白致敏激发制备豚鼠哮喘模型,分离豚鼠BALF中的EOS,肺组织切片HE染色,利用计算机图像分析仪测定支气管管壁厚度(WAt/Pi)、支气管平滑肌厚度(WAm/Pi)、支气管平滑肌细胞计数(N/Pi),酶联免疫吸附法(ELISA)检测BALF中IL-5的含量,Td T介导的d UTP缺口末端标记法(TUNEL)检测EOS凋亡。结果:模型组WAt/Pi、WAm/Pi、N/Pi均较高于正常组(P<0.01),中药组WAt/Pi、WAm/Pi、N/Pi均低于模型组(P<0.05)。模型组IL-5水平高于正常组(P<0.01),中药组IL-5水平低于模型组(P<0.05)。模型组豚鼠EOS凋亡明显减少,中药组EOS凋亡明显高于模型组(P<0.01)。结论:中药补肺益肾方可通过降低气道IL-5水平,促进EOS凋亡,抑制气道重塑,减轻气道炎症,达到防治哮喘的目的。

Effects of Traditional Chinese Medicine of Invigorating Lung and Nourishing Kidney on EOS Apoptosis in Airway Remodeling of Young Guinea Pigs with Asthma

Objective ： To explore the effects of the traditional Chinese medicine of invigorating lung and nourishing kidney on airway remodeling,interleukin - 5 （ IL - 5） level and eosinophils （EOS） apoptosis of bronchoalveolar lavage fluid （BALF） of young guinea pigs with asthma. Methods：24 young guinea pigs were divided into three groups randomly, normal control group, model group and the traditional Chinese medicine group. Asthma model of guinea pigs were sensitized by ovalbumin. EOS was pu- rified from BALF. Lung tissue sections were stained with HE. The airway wall thickness （ WAt/Pi）, bronchial smooth muscle thickness （WAm/Pi） and bronchial smooth nucleus count （N/Pi） were measured by using computer- assisted image analysis system. The content of IL -5 in BALF was detected by enzyme - linked immunosorbent assay （ELISA）. Apoptosis was detected by TdT- mediated dUTP nick end labeling （TUNEL）. Results：The WAt/Pi, WAm/Pi and N/Pi in model group were all higher than those of normal group（ P 〈0. 01 ） while the WAt/Pi ,WAm/Pi and N/Pi in the traditional Chinese medicine group were all lower than those of model group （ P 〈 0. 05 ）. The content of IL - 5 in model group was higher than that of normal group （ P 〈 0.01 ） while the content of IL - 5 in the traditional Chinese medicine group was lower than that of model group （ P 〈 0. 05 ）. EOS apoptosis in model group guinea pig was significantly decreased while EOS apoptosis in the traditional Chinese medicine group guinea pig was higher than that of model group （P 〈 0. 01 ）. Conclusion：The traditional Chinese medicine of invigorating lung and nourishing kidney relieves airway inflammation and suppresses airway remodeling by decreasing the content of IL - 5 in airway and promoting EOS apoptosis. So it can prevent asthma.