针刀疗法对膝骨关节炎兔关节软骨细胞凋亡的影响

目的:观察针刀疗法治疗膝骨关节炎兔对其细胞凋亡的影响,探讨可能的作用机制。方法:正常新西兰兔随机分为:正常组、模型组、针刀组、中药组。左后膝伸直位石膏管型固定6周建立膝骨关节炎兔模型,原位末端标记(TUNEL)检测关节软骨细胞凋亡。结果:细胞凋亡指数正常组为(4.9±1.7),模型组为(25·2±4.3),针刀组为(11.6±3.1),中药组为(19.1±3.4)。模型组细胞凋亡指数显著升高,与正常组比较,经统计学处理,差异具有显著性意义(P<0.01)。针刀治疗和中药治疗均能降低细胞凋亡指数,两组比较,差异具有显著性意义(P<0.01)。结论:针刀疗法能抑制膝骨关节炎兔关节软骨细胞的凋亡,促进关节软骨形态恢复,这可能是针刀治疗膝骨关节炎的作用机制之一。

Effect of Acupotomy on Apoptosis in the Rabbit of Knee Osteoarthritis

Objective:To investigate the effect of acupotomy on apoptosis in the rabbit of Knee Osteoarthritis and explore the possible mechanism. Methods:Adult rabbits were randomly assigned into normal group,model group, Acupotomy group,and Chinese medicine group.The models of KOA were established by joint immobilization with plaster bandage for six weeks.Obseryc apoptosis with TUNEL in every group. Result Apoptosis rate in normal group,model group,Acupotomy group,and Chinese medicine group were 4.9±1.7,25.2±4. 3, 11.6±3.1,and 19.1±3.4 respectively.The Apoptosis rate in model group was significantly higher than that of normal group, and the difference was Sigriificant by statistical analysis (P<0.01) . Apoptosistherapy and Chinese medicine therapy could decmase the Apoptosis rate, and there was statistical difference between these two groups (P<0.01) . Conclision: Acupotomy may decrease the apoptosis and induce the recovery of the articular cartilage for treating KOA .This may be one of the mechanisms of Acupotomy for KOA.