加味四逆散含药血清对HSC凋亡影响的实验研究

目的:观察加味四逆散含药血清对大鼠肝星状细胞(HSC-T6)凋亡的影响。方法:以不同浓度的加味四逆散含药血清作用于体外培养的HSC-T6细胞,分别作用12h、24h、48h后,采用流式细胞仪和TUNEL法检测加味四逆散对HSC-T6凋亡的影响。结果:(1)流式细胞仪分析结果显示:加味四逆散各浓度组、复方鳖甲软肝片组与HSC作用12h、24h、48h后,细胞凋亡与空白对照组比较均有差异(P<0.05);与鳖甲软肝片组比较,除加味四逆散低浓度组作用12h时细胞凋亡无差异外(P>0.05),其余各浓度组细胞凋亡均优于鳖甲软肝片组,差异有统计学意义(P<0.05);(2)TUNEL检测表明:加味四逆散各浓度药物血清作用48h后,细胞凋亡率与空白对照组及鳖甲软肝片组药物血清比较差异均有统计学意义(P<0.05)。结论:加味四逆散含药血清可显著促进HSC-T6凋亡,且以高剂量和作用48h最为明显。

Experimental Study of Effect of JWSNS Serum on Apoptosis of HSC-T6

Objective:To observe the effect of JWSNS serum on apoptosis of hepatic stellate cells.Methods:Different concentrations of JWSNS Decoction acted on drug-containing serum in vitro HSC-T6 cells,using flow cytometry and TUNEL method for measuring HSC-T6 apoptosis after 12h,24h,48h.Results:Flow cytometry analysis showed that it had differences when each concentration group compared with the control group(P<0.05)after 12h,24h,48h.It had differences when each concentration group of JWSNS compared to Biejia Tuangan tablet(P<0.05)in addition to low concentration of 12h(P>0.05).(2)TUNEL method showed that it had differences when each concentration group compared with the control group after 48h(P<0.05).Conclusion:JWSNS can promote the apoptosis of HSC-T6 and the role of high dose and 48h was obvious.