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| 补阳还五汤对内皮型eNOS启动子活性的影响 | |
| 引用本文： | 孙学刚,张丽华,蔡红兵,赵益业,蔡宇.补阳还五汤对内皮型eNOS启动子活性的影响[J].山东中医杂志,2005,24(3):162-165. |
| 作者姓名： | 孙学刚 张丽华 蔡红兵 赵益业 蔡宇 |
| 作者单位： | 1. 南方医科大学,中医系,广东,广州,510515 2. 广东省中医院,心脏中心,广东,广州,510405 3. 暨南大学药学院,广东,广州,510632 |
| 基金项目： | 广东省中医药管理局科研课题(1040088) |
| 摘 要： | 目的:研究补阳还五汤含药血清对人内皮型一氧化氮合酶(eNOS)启动子活性的影响。方法:基因亚克隆方法构建pGL2 eNOS和pEGFP- 1 -eNOS质粒载体;应用jetPEITM转染内皮细胞ECV304,分别用HTS7000生化分析仪和荧光显微镜观察报告基因的表达。结果:含补阳还五汤及黄芪的血清均能增强 pEGFP -1- eNOS转染的内皮细胞的绿色荧光蛋白的表达,并能增强转染 pGL2- eNOS荧光素酶的表达。结论:补阳还五汤和黄芪可能通过增强内皮型eNOS启动子的活性、延缓内皮功能障碍的发生以防治动脉粥样硬化。 |
| 关 键 词： | 补阳还五汤 内皮型一氧化氮合酶 启动子 报告基因 动脉粥样硬化 |
| 文章编号： | 0257-358X(2005)03-0162-04 |
| 修稿时间： | 2004年12月15 |
Effects of buyang huanwu decoction on the promotor activity of endothelial nitric oxide synthase promotor |
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| SUN Xue-gang,ZHANG Li-hua,CAI Hong-bing,ZHAO Yi-ye,CAI Yu.Effects of buyang huanwu decoction on the promotor activity of endothelial nitric oxide synthase promotor[J].Shandong Journal of Traditional Chinese Medicine,2005,24(3):162-165. | |
| Authors: | SUN Xue-gang ZHANG Li-hua CAI Hong-bing ZHAO Yi-ye CAI Yu |
| Institution: | SUN Xue-gang1,ZHANG Li-hua1,CAI Hong-bing1,ZHAO Yi-ye2,CAI Yu3 |
| Abstract: | Objective: to study the effects of buyang huanwu decoction on the promotor activity of endothelial nitric oxide synthase (eNOS). Methods: pGL2-eNOS and pEGFP-1-eNOS were constructed by subclone method. The activity of pGL2-eNOS was measured by HTS 7000 Bio assay reader and the expression of pEGFP-1-eNOS was observed by fluorescence microscope. Results: the activity of both pGL2-eNOS and pEGFP-1-eNOS increased after treated by buyang huanwu decoction containing serum and astragalus membranaceus containing serum in ECV304 cells. Conclusion: buyang huanwu decoction increased the activity of the eNOS promotor. It must be the mechanism of buyang huanwu decoction in preventing the endothelial dysfunction. |
| Keywords: | buyang huanwu decoction eNOS promotor reporter gene atheroslerosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |