| HPLC转换波长法测定木瓜中绿原酸、齐墩果酸和熊果酸含量 |
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| 引用本文： | 齐,红,郭庆梅,李圣波,周凤琴.HPLC转换波长法测定木瓜中绿原酸、齐墩果酸和熊果酸含量[J].山东中医杂志,2014(3):227-229. |
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| 作者姓名： | 齐 红 郭庆梅 李圣波 周凤琴 |
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| 作者单位： | [1]山东中医药大学2012年级博士研究生,山东济南250355 [2]济南市食品药品检验检测中心,山东济南250001 [3]山东中医药大学,山东济南250355 [4]山东亚特生态技术有限公司,山东临沂266071 |
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| 摘 要： | 目的:建立HPLC转换波长法,同时测定木瓜中绿原酸、齐墩果酸、熊果酸含量。方法 :采用HPLC法,Kromasoil C18(250 mm×4.6 mm,5μm)色谱柱,流动相:乙腈-0.6%磷酸溶液,梯度洗脱,流速:0.8 mL/min,检测波长:326 nm,210 nm;柱温:30℃。结果:绿原酸在0.192~2.880μg(r=0.9994),齐墩果酸在0.488~7.320μg(r=0.9999),熊果酸在0.437~6.468μg(r=1)范围内,线性关系良好;三种化合物的平均回收率为别为101.13%(RSD=1.98%,n=6)、99.64%(RSD=1.97%,n=6)及99.66%(RSD=1.90%,n=6)。结论:该方法简便准确,重现性好,可为控制木瓜质量提供参考。
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| 关 键 词： | 木瓜 转换波长法 HPLC 绿原酸 齐墩果酸 熊果酸 |
Determination of Chlorogenic Acid、Oleanolic Acid and Ursolic Acid in Fructus Chaenomelis by Wavelength Conversion-HPLC |
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| QI Hongl＇Z,GUO Qingmeil,LI Shengbo,ZHOU Fengqin.Determination of Chlorogenic Acid、Oleanolic Acid and Ursolic Acid in Fructus Chaenomelis by Wavelength Conversion-HPLC[J].Shandong Journal of Traditional Chinese Medicine,2014(3):227-229. |
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| Authors: | QI Hongl＇Z GUO Qingmeil LI Shengbo ZHOU Fengqin |
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| Institution: | 1 (1.Postgraduate of 2012 Grade of Shandong University of Traditional Chinese Medicine,Jinan 250355,China; 2.Jinan Institute for Food and Drug Control,Jinan 250001 ,China;3.Shandong University of Traditional Chinese Medicine,Jinan 250355, China;4.Shandong Yate Eeo-tech Co.Ltd, Linyi 266071,China) |
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| Abstract: | Objective：To estab1ish a wave1ength conversion method of HPLC for simu1taneous determination of ch1orogenic acid,o1eano1ic acid and urso1ic acid in Fructus Chaenome1is. Methods：Stationary phase was Kro masoi1 C1s（250mm×4.6mm,5 μm） with the co1umn temperature of 30 ℃. Acetonitri1-0.6% H3PO4 was used as the mobi1e phase with gradient e1ution. The f1ow rate was 0.8 mL/min. The detection wave1ength was 326 nm and 210 nm. Resu1ts：The ch1orogenic acid had a good 1inearity re1ationship（r = 0.9994,n = 6） between 0.192N2.880 μg. The o1eano1ic acid had a 1inear range between 0.488-7.320 μg with r=0.9999（n=6）. The urso1ic acid had a 1inear range between 0.437-6.468 μg with r= 1（n=6）. The average recovery of ch1orogenic acid was 101.13% with RSD of 1.98%,the o1eano1ic acid was 99.64% with RSD of 1.97%,the urso1ic acid was 99.66% with RSD of 1.90%. Conc1usion：This method is simp1e,accurate,reproducib1e and can be used for the qua1ity contro1 of Fructus Chaenome1is. |
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| Keywords: | Fructus Chaenomelis Wavelength Conversion method HPLC Chlorogenic acid Oleanolic acid Ur-solic acid |
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