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炎症性肠病患者肠道菌群结构分析
引用本文:戎建明,王少东,汪芳裕.炎症性肠病患者肠道菌群结构分析[J].现代中西医结合杂志,2012,21(25):2750-2752.
作者姓名:戎建明  王少东  汪芳裕
作者单位:南京军区南京总医院,江苏,南京,210002
摘    要:目的应用PCR-DGGE技术对炎症性肠病患者肠道微生物菌群结构及其相似性、多样性进行研究。方法选择正常对照者9例(对照组)和炎症性肠病患者9例(实验组),采集各自粪便,提取细菌总基因组DNA,PCR扩增细菌16S rDNA基因V6—8可变区,DGGE方法检测PCR产物,对肠道菌群进行指纹图谱分析。结果正常对照组和炎症性肠病组PCR-DGGE指纹图谱分析显示,炎症性肠病细菌的多样性较正常对照组明显下降。结论PCR-DGGE技术是一种快速有效的用于分析研究人体肠道菌群结构的技术。阴道菌群的改变可能与炎症性肠病的发生、发展密切相关。

关 键 词:炎症性肠病  PCR-DGGE  肠道菌群

Structure analysis of intestinal microbiota in patients with inflammatory bowel disease
Rong Jianming , Wang Shaodong , Wang Fangyu.Structure analysis of intestinal microbiota in patients with inflammatory bowel disease[J].Modern Journal of Integrated Chinese Traditional and Western Medicine,2012,21(25):2750-2752.
Authors:Rong Jianming  Wang Shaodong  Wang Fangyu
Institution:(Nanjing General Hospital of Nanjing Military Command, Nanjing 210002, Jiangsu, China)
Abstract:Objective It is to detect and analyze the microbial composition and its diversity, similarity by denaturing gradient gel electrophoresis(DGGE) in patients with inflammatory disease (IBD). Methods 9 normal controls (control group) and 9 patients with IBD (experimental group) were enrolled in the study. Each group include 9 samples. Their faeces were collected to detect the microbiological profiles V6_8 region of the 165 rDNA gene of all bacteria, and they was amplified by PCR and separated by DGGE. Results PCR - DGGE fingerprinting analysis of both groups showed that the diversity of intestinal microbiota of experimental group was significantly lower than that in control group. Conclusion PCR - DGGE is a technique which can lastly and effectively analyze and study human whole intestinal microbial communities. The changes of intestinal microbiota might be closely related with occurrence and development of IBD.
Keywords:inflammatory bowel disease  PCR - DGGE  gut microbiota
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