子宫内膜异位症患者血管内皮生长因子的测定

目的　探讨子宫内膜异位症 (内异症 )患者腹腔液及血清中血管内皮生长因子(VEGF)在内异症发病中的作用。方法　采用双抗体夹心酶联免疫吸附法 (ELISA) ,检测 35例内异症患者 (Ⅰ期和Ⅱ期 16例 ,Ⅲ期和Ⅳ期 19例 )和 2 0例非内异症患者 (对照组 )腹腔液及血清中VEGF的含量 ,分析VEGF浓度及其与内异症分期R -AFS的相关性。结果　内异症患者腹腔液及血清中VEGF的浓度明显高于对照组 (P <0 .0 1) ,2组腹腔液VEGF含量均高于同组的血清含量 (P <0 .0 5 ) ;Ⅲ期和Ⅳ期内异症患者腹腔液及血清VEGF含量明显高于Ⅰ期和Ⅱ期患者 (P <0 .0 1) ,其中增生期患者又明显高于分泌期患者 (P <0 .0 5 ) ;而内异症患者R -AFS分期与腹腔液及血清VEGF含量无明显相关 ,腹腔液与血清VEGF含量无相关。结论　内异症患者腹腔液中VEGF升高是腹腔微环境改变导致内异症的原因。

Measurement of vascular endothelial growth factor levels in patients with endometriosis

Objective It is to discuss the role of vascular endothelial growth factor (VEGF) in ascitic fluid and serum on the pathogenesis of endometriosis (EM).Methods VEGF contents in ascitic fluid and serum of 35 patients with EM (study group) and 20 patients without EM (control group) were measured with enzyme linked immunoabsorbent assay (ELISA). The VEGF concentration and the relation with EM stage (R-AFS) were analyzed. Results The VEGF concentration in ascitic fluid and serum of study group were obviously higher than that of control group (P<0.01). In each group, the VEGF concentration in ascitic fluid was higher than that in serum (P<0.05). The VEGF concentrations in ascitic fluid and serum in stage 3 and 4 were both obviously higher than that in stage 1 and 2 (P<0.01). The VEGF concentration in hyperplastic stage was obviously higher than that in excretive stage (P<0.05). There was no obviously relation between R-AFS and VEGF in ascitic fluid and serum, and no relation between VEGF in ascitic fluid and that in serum.Conclusion The VEGF increasing of patients with EM may cause the changing of the celiac microenvironment which leads to EM.