实时荧光定量聚合酶链反应法检测肺结核患者痰标本价值的对照研究

目的探讨不同方法对肺结核的诊断价值。方法以实时荧光定量聚合酶链反应法、痰直接涂片找抗酸杆菌法、痰结核杆菌培养法对我院56例确诊肺结核患者痰标本检查比较。结果荧光定量PCR法检出结核杆菌阳性率显著高于痰涂片抗酸染色和培养法,其他非肺结核结果阳性率仅为2.7%,特异度较高。结论实时荧光定量聚合酶链反应法对肺结核诊断方面灵敏度及特异度较高,同时反映抗结核治疗过程中痰标本中的结核杆菌的数量变化,对抗结核药物疗效有良好的监控效果,应列入肺结核常规实验室检查项目。

Compared study of sputum specimen from patient with pulmonary tuberculosis detected with fluorogenic probes and real-teme PCR

Objective It is to discuss the diagnosis value of different methods on pulmonary tuberculosis.Methods The sputum specimens obtained from 56 patients with tuberculosis were detected Mycobacterium tuberculosis(MTB) with fluorogenic probes and real-time PCR(FQ-PCR),smear acidfast and culture of organism.Results The positive rate of MTB detected with FQ-PCR was higher than that with smear acid-fast and culture of organism.The positive rate of non-tuberculosis was only 2.7%.The specificity was higher.Conclusion The high sensitivity and specificity rate renders a positive FQ-PCR result,which is clinically useful.Furthermore,the variation of the TB-DNA reflects the variation of the Mycobacterium tuberculosis when treatment.It is useful to monitor the curative effect.It should be a routine test to detect pulmonary tuberculosis.