简易悬吊术制备大鼠深静脉血栓模型

目的建立一种简便、适合动态研究的大鼠深静脉血栓模型。方法将60只体质量为(250±20)g的SD大鼠按随机数字表法分为3组:动物模型组36只,对照组18只,假手术组6只。动物模型组采用改良下腔静脉结扎法(简易悬吊术)制备动物模型,造模后观察血管内膜及血栓不同时期的病理变化,并与对照组及假手术组做观察比较。结果采用改良法制作模型后,大鼠下腔静脉内不仅均形成稳定的血栓,而形成血栓的大鼠存活率很高,符合人体静脉血栓形成急性期的病理演变。结论简易悬吊术可以成功制作适合动态研究的大鼠深静脉血栓模型。

Deep venous thrombosis model in rats prepared with simple and easy ventrofixation

Objective It is to establish a kind of convenient deep venous thrombosis(DVT) model in rats to suit dynamic study.Methods 60 SD rats whose body mass was(250±20) g were divided into animal model group in which there was 36 rats,control group in which there was 18 rats and sham operation group in which there was 6 rats with random digits table method.Improved inferior vena cava ligation method(simple and easy ventrofixation) was used in animal model group to prepare animal model.After model prepared,the pathological changes of thrombus and endangium in different period in animal model group were observed and compared with those in control group and sham operation group.Results After model prepared with improved method,stable thrombus was formed in inferior vena cava of rats.The survival rate of rats which had thrombus formed was very high.The pathological changes of animal model were consistent with the pathological variation of venous thrombosis during acute stage in human body.Conclusion Simple and easy ventrofixation can be used to successfully establish DVT model in rats to suit dynamic study.