丹星通络汤对脑缺血再灌注损伤大鼠热休克蛋白70表达的影响

目的观察丹星通络汤对大鼠脑缺血再灌注损伤神经细胞热休克蛋白70(heatshock protein 70,HSP70)表达的影响,探讨其神经保护作用机制。方法40只健康雄性Wistar大鼠,采用大脑中动脉线栓法建立局灶脑缺血再灌注大鼠模型,并随机将大鼠分为假手术组,缺血再灌注组(I/R),丹星通络汤大、小剂量组和尼莫地平组,每组8只。丹星通络汤组分别按15mL/kg(大剂量),7.5 mL/kg(小剂量),尼莫地平组10 mg/kg,术前连续给药7 d,每天1次。假手术组和缺血再灌组分别灌以10 mL/kg的生理盐水。大鼠局灶性脑缺血2 h,然后行再灌注。再灌注24 h断头取脑组织,采用免疫组织化学法检测脑组织中HSP70蛋白的表达,并与病理组织学改变进行对照。结果丹星通络汤能明显增强大脑皮质及海马HSP70的表达,减轻神经细胞损伤。结论丹参通络汤能减轻脑组织的缺血再灌注损伤,改善神经功能缺失,其作用机制可能与增加HSP70表达有关。

Influence of Danxingtongluo decoction on heat shock protein 70 expression in rats with cerebral ischemia reperfusion injury

Objective It is to observe the influence of Danxingtongluo decoction(DXTLD) on nerve cell heat shock protein 70(HSP70) expression in rats with cerebral ischemia reperfusion injury and to discuss its nerve protection mechanism.Methods Forty healthy male Wistar rats were chosen to establish focal cerebral ischemia reperfusion models with middle cerebral artery thread embolism method and were randomly divided into sham operation group,ischemia/reperfusion(I/R) group,DXTLD high dosage group,DXTLD low dosage group and Nimodipine(NIMO) group. There were eight rats in every group.DXTLD groups were given DXTLD at doses of 15 mL/kg and 7.5 mL/kg.NIMO group was given NIMO at dose of 10 mg/kg.The drugs were given once a day and consecutively for seven days before operation.Normal saline were given to sham operation group and I/R group at dose of 10 mL/kg.After 2 h ischemia and 24 h reperfusion injury,the rats were killed and their brains were taken out.The HSP70 expression in brain tissue was measured with immunohistochemical method and was compared with the change of pathohistology.Results DXTLD could obviously increase the HSP70 expression in cerebral cortex and hippocampus and relieve nerve cell injury.Conclusion DXTLD can relieve ischemia reperfusion injury of brain tissue and improve nerve function lose.The mechanism may be related with the increasing of HSP70 expression.