CD_(19)~+CD_(23)~+在婴幼儿支气管哮喘淋巴细胞上的表达及临床意义

目的探讨婴幼儿支气管哮喘B淋巴细胞表面CD19+CD23+的表达及临床意义。方法采用流式细胞分析技术及免疫化学发光法检测32例哮喘发作期患儿(哮喘发作组)和29例哮喘缓解期患儿(哮喘缓解组)外周血B淋巴细胞CD19+CD23+表达率和血清总IgE水平,并与23例健康体检者(正常对照组)对照。结果哮喘发作组B淋巴细胞CD19+CD23+及血清IgE水平均高于哮喘缓解组和正常对照组(P<0.01);哮喘缓解组CD19+CD23+的表达率明显高于正常对照组(P<0.01);哮喘发作组CD19+CD23+表达率与血清IgE呈正相关(P<0.01)。结论外周血B淋巴细胞表面CD19+CD23+检测有助于婴幼儿支气管哮喘的诊断和预后判断。

Expression of CD19+ and CD23+ on B lymphocytes in infantile bronchial asthma and its clinical significance

Objective It is to explore the expression of CD_(19)~ and CD_(23)~ on B lymphocytes in infantile bronchial asthma and its clinical significance.Methods Expression of CD_(19)~ and CD_(23)~ on B lymphocytes in peripheral blood and total serum IgE were analyzed with flow cytometry(FACS) and immuno-chemiluminescence in 32 infants with bronchial asthma in period of onset and 29 ones at remission stage.At the same time,23 age matched healthy infants were chosen as normal control.Results The levels of CD_(19)~ and CD_(23)~ on B lymphocytes and total serum IgE in bronchial asthma infants in period of onset were all higher that those both in the remission stage group and normal control group(P<0.01).and the expression rate of CD_(19)~ and CD_(23)~ on B lymphocytes in remission stage group was obviously higher than that in the control group(P<0.01).Furthermore, a positive relationship could be found between CD_(19)~ and CD_(23)~ on B lymphocytes and serum IgE in bronchial asthma Infants.Conclusion Analysis of CD_(19)~ and CD_(23)~ on B lymphocytes is helpful for diagnosis and prognosis of the infantile bronchial asthma.