| 复合蟾毒灵的壳聚糖/纳米羟基磷灰石人工骨对骨肉瘤细胞增殖的影响 |
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| 引用本文： | 陈黄琴,黄彬.复合蟾毒灵的壳聚糖/纳米羟基磷灰石人工骨对骨肉瘤细胞增殖的影响[J].现代中西医结合杂志,2011,20(25):3143-3144,3146. |
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| 作者姓名： | 陈黄琴 黄彬 |
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| 作者单位： | 咸宁学院,湖北,咸宁,437100 |
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| 摘 要： | 目的探讨复合蟾毒灵的壳聚糖/纳米羟基磷灰石人工骨对骨肉瘤细胞增殖的影响,希望为骨肉瘤的化疗和修复重建找到新的思路。方法冷冻干燥法制备复合人工骨,通过体外模拟液浸泡实验观察蟾毒灵在体外缓释情况;激光共聚焦显微镜观察细胞三维生长分布的状态;MTT法检测复合人工骨对骨肉瘤细胞增殖的影响。结果蟾毒灵有效释药时间为16 d;细胞在支架上多为游离分布,球形,核固缩;并且从培养的第4天开始含100mg/L蟾毒灵组细胞抑制率>含50 mg/L蟾毒灵组>含25 mg/L蟾毒灵组。结论复合蟾毒灵的壳聚糖/纳米羟基磷灰石人工骨能够长期持续抑制骨肉瘤细胞的增殖,可能为临床治疗骨肉瘤提供一种新的思路。
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| 关 键 词： | 蟾毒灵 支架 骨肉瘤细胞 增殖 |
Effect of Bufalin/chitosan/nano-hydroxyapatite scaffolds on proliferation of osteosarcoma cells |
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| Chen Huangqin,Huang Bin.Effect of Bufalin/chitosan/nano-hydroxyapatite scaffolds on proliferation of osteosarcoma cells[J].Modern Journal of Integrated Chinese Traditional and Western Medicine,2011,20(25):3143-3144,3146. |
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| Authors: | Chen Huangqin Huang Bin |
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| Institution: | Chen Huangqin,Huang Bin(Xianning College,Xianning 437100,Hubei,China) |
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| Abstract: | Objective It is to explore the effect of Bufalin/chitosan/nano-hydroxyapatite(bufalin/CS/nano-HA) scaffolds on proliferation of human osteosarcoma cells in vitro,hope to find novel approaches of chemotherapy and reconstruction for osteosarcoma.Methods Bufalin/CS/nano-HA scaffolds were prepared by freezing and lyophylization of composite solution.Simulated body fluid(SBF) soaking test in vitro was used to investigate the drug release.Confocal laser scanning microscopy(CLSM) was used to observe 3-dimensional ... |
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| Keywords: | bufalin scaffold osteosarcoma cells proliferation |
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