| 微乳薄层色谱定性鉴别蒲公英中黄酮类成分的研究 |
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| 引用本文： | 赵惠茹,蒋礼杰.微乳薄层色谱定性鉴别蒲公英中黄酮类成分的研究[J].现代中药研究与实践,2014(3):20-22. |
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| 作者姓名： | 赵惠茹 蒋礼杰 |
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| 作者单位： | 西安医学院药学院,陕西西安710021 |
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| 基金项目： | 陕西省教育厅科研计划项目(No.12JK1024) |
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| 摘 要： | 目的建立蒲公英药材中黄酮类成分的微乳薄层色谱定性鉴别方法。方法以黄酮为目标成分,十二烷基硫酸钠-正丁醇-正庚烷-水组成的微乳体系为展开剂,对蒲公英提取物进行聚酰胺薄层层析,考察微乳液类型和酸度对分离效果的影响。结果以含水量70%微乳液-甲酸(9.0∶1.0)为适宜的展开剂,与常规展开剂相比,分离得到的斑点多,且斑点清晰、圆整。结论微乳薄层色谱对蒲公英黄酮类成分的分离效果较好,可用于蒲公英药材中黄酮成分的定性鉴别。
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| 关 键 词： | 蒲公英 黄酮 微乳液 薄层色谱 |
Identification of Flavonoids in Taraxacum Herb by Microemulsion Thin-Layer Chromatography |
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| ZHAO Hui ru,JIANG Li jie.Identification of Flavonoids in Taraxacum Herb by Microemulsion Thin-Layer Chromatography[J].Research and Practice on Chinese Medicines,2014(3):20-22. |
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| Authors: | ZHAO Hui ru JIANG Li jie |
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| Institution: | (Department of Pharmacy, Xi＇an Medical College, Xi＇an 710021,China) |
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| Abstract: | Objective To establish the microemulsion thin-layer chromatography for identifying fl avonoids in Taraxacum Herb.Methods With the fl avonoids as target component,the samples were eluted by the developer of SDS / n-C4H9OH / n-C7H16 /H2O.The infl uence of the type of microemulsion and pH were investigated through polyacetamide thin-layer chromatography.Results The optimum developer of microemulsion contains 70% water- formic acid（9.0：1.0）.Spots were clear and round comparing with the other developers.Conclusion The separation effect by microemulsion thin-layer chromatography was improved signifi cantly,the method can be used for the identifi cation of fl avonoids in Taraxacum Herb. |
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| Keywords: | Taraxacum Herb flavonoids microemulsion thin-layer chromatography |
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