高效液相色谱法测定藤黄药材中藤黄酸与新藤黄酸的含量

目的建立藤黄药材中藤黄酸与新藤黄酸的含量测定方法。方法采用反相高效液相色谱法,色谱柱为Purospher RP-18(250mm×4.6mm,5μm);流动相:乙腈A-0.1%乙酸水溶液B(0-30min80%-90%A,30-32min90%-80%A);流速:1ml/min;检测波长为360nm,外标法定量。结果藤黄酸的线性范围为0.0408~0.4080mg/m L,回归方程Y=16023031X-54083(r=0.9991,n=5),平均回收率为99.7%,RSD=1.39%(n=9);新藤黄酸的线性范围为0.0372~0.3720mg/m L,回归方程Y=11893364X-34797(r=0.9999,n=5),平均回收率为100.1%,RSD=1.00%(n=9)。结论本方法精密度高,重复性好,简便、可靠,可作为藤黄中藤黄酸和新藤黄酸的含量检测方法。

Determination of the Content of Gambogic Acid and Neo-gambogic Acid in Gamboge by HPLC

Objective To establish a HPLC method to determinate the content of Gambogic acid and Neo- gambogic acid in Gamboge. Methods The column of RP-HPLC was Purospher RP-18（250mm×4.6mm,5μm）. The mobile phase was consisted of acetonitrile A-water （0.1% Acetic acid） B （0-30min80%-90%A, 30-32min90%- 80%A）, the wavelength of detector was 360 nm, at a flow rate of 1.00 mL/min. Both were assayed with external standard method. Results The method of Gambogic acid was linear at the range of 0.040 8～0.408 0mg/mL with the regression equation Y= 16 023 031X-54 083（r=0.999 1, n=5）, the average recovery was 99.7%, RSD wasl.39% （n=9）. The method of Neo-gambogic acid was linear at the range of 0.037 2～0.372 0mg/mL with the regression equation Y=ll 893 364X-34797（r=0.999 9, n=5）, the average recovery was 100.1%, RSD was 1.00%（n=9）. Conclusion The method is simple, accurate and reliable for the determination of the content of Gambogic acid and Neo-gambogic acid in Gamboge, and can be used for quality control of Gamboge.