| 3个仙草品系核糖体DNA-ITS序列分析 |
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| 引用本文： | 黄玉吉,黄颖桢,陈菁瑛,苏海兰.3个仙草品系核糖体DNA-ITS序列分析[J].现代中药研究与实践,2012(3):22-25. |
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| 作者姓名： | 黄玉吉 黄颖桢 陈菁瑛 苏海兰 |
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| 作者单位： | 福建省农业科学院农业生物资源研究所,福建福州350003 |
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| 基金项目： | 福建省公益类科研项目（2009R10037-6）; 福建省科技资源平台建设项目（2008Y2003） |
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| 摘 要： | 目的为仙草资源的分子标记和仙草品种选育提供参考。方法采用克隆测序法,测定仙草3个品系的核糖体DNA-ITS序列。结果 3个仙草品系的ITS序列存在2个碱基差异,分别在ITS1区段的第355位和在ITS2区段的第578位。结论 3个仙草品系间既有差异又有较近的亲缘关系,序列结果已登录到NC-BI。
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| 关 键 词： | 仙草 品系 rDNA-ITS序列 |
Sequence Analysis of Nuclear Ribosomal DNA of Mesona Chinensis benth. |
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| HUANG Yu-ji,HUANG Ying-zhen,CHEN Jing-ying,SU Hai-lan.Sequence Analysis of Nuclear Ribosomal DNA of Mesona Chinensis benth.[J].Research and Practice on Chinese Medicines,2012(3):22-25. |
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| Authors: | HUANG Yu-ji HUANG Ying-zhen CHEN Jing-ying SU Hai-lan |
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| Institution: | HUANG Yu-ji, HUANG Ying-zhen, CHEN Jing-ying, SU HaMan (Agricultural Bio-resources Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China) |
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| Abstract: | Objective In order to provide a recommendable reference for the research on the molecular marker and breeding of Mesona chinensis Benth.. Methods The nuclear ribosomal DNA fragment was cloned and se- quenced. Results The results showed that ITS sequences of three different strains had two bases difference, 355 in ITS1 and 578 in ITS2, respectively. Conclusion A close relationship existed among the three strains of Mesona chinensis Benth. , and the rDNA ITS sequences obtained were submitted to GenBank. |
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| Keywords: | Mesona chinensis benth strain rDNA-ITS sequence |
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