白花蛇舌草多糖对人宫颈癌Hela细胞和人肝癌Bel-7402细胞生长的影响

目的研究白花蛇舌草多糖对体外培养的人肝癌Bel-7402细胞和人宫颈癌Hela细胞生长的影响。方法常规法体外培养人肝癌Bel-7402细胞和人宫颈癌Hela细胞,设空白对照组、阳性对照组和供试药物组(大、中、小剂量),分别采用MTT法和流式细胞仪检测白花蛇舌草多糖对人宫颈癌Hela细胞和人肝癌Bel-7402细胞的生长抑制率和凋亡率;采用倒置显微镜观察细胞形态学变化。结果白花蛇舌草多糖可抑制人肝癌Bel-7402细胞和人宫颈癌Hela细胞的生长,并促进其凋亡,其作用效果与其浓度正相关,与空白对照组相比具均有显著性差异(P﹤0.01)。结论本研究结果提示,白花蛇舌草多糖具有较好的抗肿瘤活性。

Effect of Polysaccharides of Oldenlandia diffusa on Growth of Bel-7402 and Hela Cells

Objective To study effect of polysaccharides of Oldenlandia diffusa （POD） for growth of Bel-7402 and Hela cells.Methods The Bel-7402 and Hela cells were cultivated cell culture in vitro,there were 5 groups：blank controller group,controlled drug group （high,low and middle dose）and 5-Fu group.Bel-7402 and Hela cells were dealed with them respectively.The morphologic changes of human Bel-7402 and Hela cells were observed by invert microscope.Inhibition of human Bel-7402 and Hela cells proliferation was measured by MTT assay and Flow Cytometer.Results POD could inhibit the proliferation of human Bel-7402 and Hela cells distinctly.The apoptosis rate of POD group was remarkably increased compared with that in blank controller group （P0.01）.The inhibition of POD group was evidently increased compared with that in blank controller group （P0.01）.Conclusion It was showed that this result had an ideal anti-tumor activity.