芪蛭益肺颗粒对COPD模型大鼠肺组织中气道重塑相关蛋白的影响

目的探究芪蛭益肺颗粒对COPD模型大鼠气道重塑抑制作用的作用机制。方法使用自制烟熏箱烟熏大鼠,持续烟熏28 d,于第1、11、21天气道内滴注LPS,滴药日不熏烟。实验第21天起,各组大鼠每日灌胃给与相应药物一次,连续给药40 d。免疫组化法测定肺组织中MMP-9、MMP-12及TIMP-1的蛋白表达水平。结果芪蛭益肺颗粒中、低剂量组MMP-9的表达显著下降(P <0.05);芪蛭益肺颗粒高、低剂量组MMP-12表达显著下降(P <0.05);罗红霉素组、芪蛭益肺颗粒高、低剂量组TIPM-1的表达显著下降(P <0.05)。结论芪蛭益肺颗粒可通过调节金属蛋白酶-抗金属蛋白酶体系的动态平衡,产生抑制COPD模型大鼠气道重塑的作用。

Effects of Qizhi Yifei Granules on Expression Level of Airway Remodeling Associated Proteins in Lung Tissue of COPD Rats

Objective To investigate the mechanism of Qizhi Yifei Granules on airway remodeling in COPD rats. Method Wistar rats are randomly divided into 6 groups: vehicle control group, model control group, roxithromycin group, and three dosage groups of Qizhi Yifei Granules. A rat model of COPD is established by infusion LPS with cigarettes smoke exposure. Rats exposed to smoke once a day for 28 days.LPS is injected into the airways on the 1 st, 11 th and 21 st day. From the 21 st day of the experiment, each group rats are administered the specific drugs once a day for 40 days. On the 21 st day, the expression levels of MMP-9, MMP-12 and TIMP-1 in lung tissue are measured with immunohistochemistry. Results Compared with the model group, the expression levels of MMP-9 are significantly decreasedin the groups of Qizhi Yifei granules at 9.10 and 4.55 g/kg(P < 0.05). The expression levels of MMP-12 are significantly decreased in the groups of Qizhi Yifei granules at 18.20 and 4.55 g/kg(P < 0.05). The expression level of TIMP-1 is significantly decreased in the roxithromycin group and groups of Qizhi Yifei granules at 18.20 and 4.55 g/kg(P < 0.05). Conclusion Qizhi Yifei Granules could inhibit airway remodeling in COPD rats by regulating the homeostasis of the metalloproteinase-antimetalloproteinase system.