| 尖瓣过路黄中槲皮素和山柰酚的定性定量分析 |
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| 引用本文： | 王岩,宋良科,董关涛,汤昊,何海洋.尖瓣过路黄中槲皮素和山柰酚的定性定量分析[J].现代中药研究与实践,2011(3):25-27. |
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| 作者姓名： | 王岩 宋良科 董关涛 汤昊 何海洋 |
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| 作者单位： | 西南交通大学生命科学与工程学院; |
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| 基金项目： | 药用植物种质资源标准化整理、整合-九节龙(2005DKA21000) |
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| 摘 要： | 目的 建立TLC及HPLC法定性定量测定尖瓣过路黄中槲皮素和山柰酚.方法 TLC法确定黄酮类化合物的种类,HPLC法测定山柰酚、槲皮素的含量.结果 槲皮素在0.081 6-0.571 2μg范围内线性关系良好(R2=0.999 4);平均回收率101.88%,RSD=1.572 4%,n=6.山柰酚在0.069 6-0...
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| 关 键 词： | 尖瓣过路黄 槲皮素 山柰酚 TLC HPLC |
Identification and Determination of Quercetin and Kaempferol in Lysimachia erosipetala Chen et C.M.Hu |
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| WANG Yan,SONG Liang-ke,DONG Guan-tao,TANG Hao,HE Hai-yang.Identification and Determination of Quercetin and Kaempferol in Lysimachia erosipetala Chen et C.M.Hu[J].Research and Practice on Chinese Medicines,2011(3):25-27. |
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| Authors: | WANG Yan SONG Liang-ke DONG Guan-tao TANG Hao HE Hai-yang |
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| Institution: | WANG Yan,SONG Liang-ke,DONG Guan-tao,TANG Hao,HE Hai-yang(School of Life Science and Engineering of Southwest Jiaotong University,Chengdu 610031,China) |
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| Abstract: | Objective To establish a TLC method and a HPLC method for determination of quercetin and kaempferol in Lysimachia erosipetala Chen et C.M.Hu. Methods By TLC and HPLC methods. TLC was used for the identification of the flavonoides. HPLC was used for the quantitative analysis of the two flavonoides. Result The linear range of quercetin was 0.081 6-0.571 2 gg （ RE = 0.999 4 ） , the average recovery was 101.8%, RSD--1.572 4% （n=6） ; The linear range of kaempferol was 0.069 6-0.487 2 μg （R2= 0.999 7 ） , the average recovery was 101.47%, RSD= 1.3211% （n=6）. The content of quercetin was 0.419 1% and kaempferol was 0.08454%. Conclusion The total content ofquercetin and Kaempferol in Lysimachia erosipetala Chen et C.M.Hu is 0.5%, it is outclassed the standard of Lysimachia christinae Hance that set in Chinese Pharmacopoeia 2010 English Edition. |
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| Keywords: | Lysimachia erosipetala Chen et C M Hu quercetin kaempferol TLC HPLC |
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