补肾生血法对慢性再生障碍性贫血患者骨髓ERK信号通路相关蛋白表达的影响

目的:探讨补肾生血法对CAA患者骨髓细胞外调节信号通路相关蛋白的表达变化及其意义。方法:将60例CAA患者按照中医辨证分为肾阴虚型33例,肾阳虚型27例,均予以补肾生血之中药补髓生血颗粒治疗,另取20例健康志愿者作为正常对照组。采用蛋白质印迹方法(Western-blot)观察CAA患者治疗前后骨髓单个核细胞ERK1、ERK2的蛋白水平表达。结果:CAA患者治疗前骨髓单个核细胞ERK1、ERK2均低于正常对照组(P均0.05),经补肾中药治疗后,ERK1、ERK2表达均有所上升(P均0.05);治疗后肾阳虚型的ERK1、ERK2上升程度均较肾阴虚型明显(P均0.05)。结论:CAA患者骨髓单个核细胞ERK1、ERK2均表达异常,提示ERK信号通路异常与CAA发病相关,补肾中药可能通过调节ERK信号通路相关蛋白ERK1、ERK2的表达来改善CAA骨髓造血功能,且存在中医证型的疗效差异。

The Study on the Expression of some Related Proteins of ERK Signaling Pathway in CAA Patients Bone Marrow with Reinforcing Kidney and Nourishing Blood

Objective： To observe the expression of extracellular regulated protein signaling pathway in CAA patients＇ bone marrow with Reinforcing Kidney and Nourishing Blood.Methods： 60 CAA patients were divided into two groups（kidney yang and yin deficiency type） by TCM,all of them were treated with Busuishengxue Granule,and another 20 healthy volunteers as normal control group.Use Western blot method to observe the expression of ERK1 and ERK2 in CAA patients＇ bone marrow mononuclear cells before and after treatment.Results： The expression of ERK1 and ERK2 in CAA patients＇ bone marrow mononuclear cells were lower than the normal control group（P all0.05）,and expression of ERK1 and EKR2 were both on the rise after treatment（P all0.05）,but the levels of kidney yang deficiency type were increased a higher degree than kidney yin deficiency type after treatment（P0.05）.Conclusion： The expression of ERK1 and ERK2 in CAA patients＇ bone marrow showed low levels,so it suggested that abnormal ERK signaling pathway is closely related with the pathogenesis of CAA,and Busuishengxue Granule may regulate the expression of ERK signaling pathway associated protein ERK1,ERK2 to improve the CAA bone marrow hematopoiesis,and there were efficacy differences in TCM syndrome type.