苏木治疗大鼠膜性肾病的实验研究

目的:观察中药苏木(水提取物)对大鼠膜性肾病(MN)的疗效,探讨其在膜性肾病治疗中的作用。方法:采用阳离子化牛血清白蛋白(C-BSA)复制大鼠MN模型,随机分为模型组(B组)、苏木组(C组)、雷公藤组(D组)、强的松组(E组),另设正常对照组(A组),每组10只。各治疗组分别给予苏木、雷公藤多甙片、强的松连续灌胃治疗4周。用药前后检测各组大鼠24h尿蛋白定量等,第4周末观察各组大鼠肾脏光镜下病理变化,并进行白介素18(IL-18)定量酶检测。结果:模型组血脂、24h尿蛋白定量显著升高,血浆蛋白降低明显,苏木组、雷公藤组、强的松组相关指标有好转趋势。光镜下观察模型组肾小球明显肿胀,球内细胞数增多,肾小球基底膜增厚,系膜区系膜细胞增生;各治疗组基底膜增厚不明显,损伤相对减轻。IL-18检测在治疗组表达较模型组有所减少。结论:苏木应用于大鼠膜性肾病后,血、尿化验有好转趋势,大鼠生存表现有所改善,病理及生化检测提示,苏木对大鼠膜性肾病有一定治疗作用。IL-18检测提示苏木在抑制免疫反应方面起到一定作用。

Experimental Study of Hematoxylon in Treating Membranous Nephropathy Rats

Objective ： To observe the effect of Hematoxylon （water extract） on membranous nephropathy（MN） in rats, and to clarify its significance in the treatment of membranous nephropathy. Methods ： Membranous nephropathy Wistar rats were induced by C -BSA and divided into groups randomly as follow： model group （group B）, hematoxylin group （Group C）, Tripterygium wilfordii group （Group D）, hormone group （Group E） , 10 in every group. The control group（ Group A） was additional. Treatment groups were respectively given hematoxylin （decoction extract Granule） , Tripterygium wilfordii tablets and prednisolone, fill stomach for four weeks in a row. 24 - hour urinary protein were detected before and after using drugs administration. The first four weeks after treatment the rats were sacrificed, dedicated renal tissue, renal tissue sections HE staining, observation of rats in each group of kidney pathological changes under light microscope. And conduct rat interleukinl8 （IL - 18） quantitative enzymes. Results： Model group, serum lipids, a 24 - hour urinary protein continues to rise, plasma protein sustain reduction. Hematoxylin group, Tripterygium wilfordii group, hormone group trends related indicators have improved. Light microscopic observation showed the glomerulus were swell- ing remarkably, the quantity of cells in glomerrulus increased significantly, glomerular basement membrane became thicker, mesangium mesangial cell proliferation; In the treatment groups glomerular basement membrane thickening were not obvious and injury lessened. Detection of IL - 18 expression in the treatment group was decreased compared with model group. Conclusion： Rats using Hematoxylin with membranous nephropathy, the blood and urine tests have improvemental trends, rats are imoroved by the clinical manifestations. Dathology prompts hematoxylin on membranous nephropathy rats therapeutic effect. IL -18 prompting hematoxylin at inhibiting the immune response plays a role to some extent.