御寒暖胃膏对胃癌前病变大鼠胃黏膜的影响

目的:观察御寒暖胃膏对胃癌前病变大鼠胃黏膜的影响。方法:将50只SD大鼠随机分为正常组、模型组、御寒暖胃膏贴敷胃经穴组、御寒暖胃膏贴敷对照点组和药物对照组,每组各10只。采用综合干预方法复制慢性萎缩性胃炎癌前病变大鼠模型,正常组喂饲标准饲料,不予任何处理。模型组自第21周开始,每天捆绑1 h,并予胶布贴敷腹部及双下肢,每只每天按0.001 m L·g~(-1)的剂量经口灌服纯净水。御寒暖胃膏贴敷胃经穴组、对照点组自第21周开始,每天将各组大鼠捆缚于鼠板,将膏药涂于所选部位上,每日1 h。每只每天按0.001 m L·g-1的剂量经口灌服纯净水。药物对照组给予维酶素溶液,按21.6 mg·kg~(-1)的药量给药,每日1次,每天捆绑1 h,并予胶布贴敷腹部皮肤及双下肢。肉眼下观察大鼠胃黏膜损伤指数,光镜下观察胃黏膜组织的病理变化,彩色多普勒观察胃黏膜的血流量。结果:与模型组比较,御寒暖胃膏贴敷胃经穴组和药物对照组大鼠胃黏膜损伤指数值均显著降低,差异具有统计学意义(P0.005);胃黏膜组织的病理损伤得到明显的修复、胃黏膜血流量均显著升高,差异具有统计学意义(P0.005)。御寒暖胃膏贴敷对照点组大鼠胃黏膜损伤指数值未见明显降低,差异无统计学意义(P0.005);胃黏膜组织的病理损伤未得到明显修复、胃黏膜血流量未见显著升高,差异无统计学意义(P0.005)。结论:御寒暖胃膏贴敷胃经穴可以促进慢性萎缩性胃炎癌前病变大鼠胃黏膜损伤的修复,增加胃黏膜的血流量,并且存在经脉—脏腑特异相关性。

The Influence of Cold-Removing and Stomach-Warming Ointment on Gastric Precancerous Lesions in Rats

Objective:To observe the influence of Cold-Removing and Stomach-Warming Ointment on gastric precancerous lesions in rats.Methods:50 SD rats were randomly divided into the normal group,the model group,Cold-Removing and Stomach-Warming Ointment stomach meridian points group,Cold-Removing and Stomach-Warming Ointment control point group,drug control group,with 10 rats in each group.Gastric precancerous lesions in rats models were copied by using comprehensive intervention method.The ones in the normal group were fed with standard diet without any treatment.Since the twenty-first week,the ones in the model group were tied up for one hour,with belly and lower limbs being applied,and took pure water orally with the amount of 0.001 mL· g-1 each day.Since the twenty-first week,the ones in Cold-Removing and Stomach-Warming Ointment stomach meridian points group,Cold-Removing and Stomach-Warming Ointment control point group were bounded to the rats board and applied ointment on the selected points,one hour each day.Each rat in the two groups took pure water orally with the amount of 0.001 mL · g-1 each day.The ones in drug control group were treated with Dimensional enzyme solution with the amount of 21.6 mg · kg-1 each day.They were tied up for one hour each day,their belly and lower limbs were applied ointment with adhesive tape.Then the gastric mucosal injury index was observed by naked eye,and the pathological changes of gastric mucosa were observed under light microscope,and the blood flow of gastric mucosa was observed by color Doppler.Results:Compared with the model group,indexes of gastric mucosal injury in Cold-Removing and Stomach-Warming Ointment stomach meridian points group,and drug control group were significantly reduced,the difference was statistically significant (P ＜ 0.005).Their pathological damage of gastric mucosa was significantly repaired and the gastric mucosal blood flow was significantly increased,and the differences were statistically significant (P ＜ 0.005).The index of gastric mucosal injury in Cold-Removing and Stomach-Warming Ointment stomach meridian points group was not obviously reduced,and the difference had no statistical significance (P ＞ 0.05).The pathological damage of gastric mucosa was not significantly repaired,and gastric mucosal blood flow was not significantly increased,the difference was not statistically significant (P ＞ 0.005).Conclusion:Cold-Removing and Stomach-Warming Ointment applied on stomach meridian points can promote the repair of gastric mucosul lesions in rats with chronic atrophic gastritis and precancerous lesions,increase gastric mucosal blood flow,and there are specific meridians-viscera correlations.