酸枣仁汤对DL-4-氯苯基丙氨酸所致失眠的药效学研究

目的:探讨酸枣仁汤治疗DL-4-氯苯基丙氨酸所致失眠大鼠的药理作用机制。方法:将50只大鼠随机分为正常对照组、模型组、地西泮组、酸枣仁汤大剂量组、酸枣仁汤小剂量组,每组10只,适应性喂养2 d,2 d后开始给药,并每天称体质量,大剂量组灌胃给予酸枣仁汤12 g·kg~(-1),小剂量组灌胃给予酸枣仁汤6 g·kg~(-1),地西泮组灌胃给予地西泮溶液1 mg·kg~(-1),正常对照组与模型组根据体质量灌胃等体积蒸馏水。建立DL-4-氯苯基丙氨酸(PCPA)大鼠失眠模型,观察酸枣仁汤对失眠大鼠自主活动次数的影响,Elisa法检测其脑内5-羟色胺(5-hydroxytvyptarmne,5-HT)、谷氨酸(glutamate,GLU)、伽马氨基丁酸(gamma amino butyric acid,GABA)、去甲肾上腺素(noradren dline,NE)及其代谢产物五羟吲哚乙酸(5-HIAA)的含量。结果:与模型组比较,地西泮组、酸枣仁汤大剂量组、酸枣仁汤小剂量组大鼠自主活动次数高于模型组,但差异无统计学意义(P0.05);酸枣仁汤大剂量组、酸枣仁汤小剂量组5-HT、5-HIAA、GABA、GLU、NE的含量均有明显变化,与模型组比较,差异具有统计学意义(P0.05)。结论:酸枣仁汤小剂量组可以明显改善PCPA所致失眠对大鼠的影响,主要体现在大鼠自主活动次数的增加,以及5-HT、5-HIAA等指标的恢复。

The Research into Efficacy of Decoction of Semen Ziziphi Spinosae in Treating Insomnia due to DL-4-chlorobenzene alanine of Rats

Objective:To explore the pharmacological action mechanism of Decoction of Semen Ziziphi Spinosae in treating insomnia due to DL-4-chlorobenzene alanine of rats.Methods:Fifty rats were randomly divided into the normal control group,the model group,the diazepam group,the group of large-dosage Decoction of Semen Ziziphi Spinosae,and the group of small-dosage Decoction of Semen Ziziphi Spinosae,with 10 cases in each group.They were fed adaptively for 2 days,then given medicine and their mass was measured each day.The ones in the group of large-dosage Decoction of Semen Ziziphi Spinosae were given 12 g · kg-1 of Decoction of Semen Ziziphi Spinosae for gavage,while the ones in the group of small-dosage Decoction of Semen Ziziphi Spinosae were given 6 g·kg-1 of Decoction of Semen Zizipbi Spinosae,the ones in the diazepam group were given 1 mg · kg-1 of diazepam,the ones in the normal control group,the model group were given the same volume of distilled water through administration by gavage according to body mass.The model of rats' insomnia due to DL-4-chlorobenzene alanine was established.Then the influence of Decoction of Semen Ziziphi Spinosae on the number of independent activities of rats with insomnia was observed.The contents of 5-HT,5 HIAA,GABA,GLU,NA of the brain were detected by using Elisa method.Results:Compared with the model group,the number of independent activities of rats in the diazepam group,the group of large-dosage Decoction of Semen Ziziphi Spinosae,and the group of small-dosage Decoction of Semen Ziziphi Spinosae,was higher than that of the model control group,but there were no significant differences between them (P＞0.05).The contents of 5-HT,5 HIAA,GABA,GLU,NA of the brain in the group of large-dosage Decoction of Semen Ziziphi Spinosae,and the group of small-dosage Decoction of Semen Ziziphi Spinosae had obvious changes,but compared with the model group,the differences between them had statistical significance (P＜0.05).Conclusion:The group of small-dosage Decoction of Semen Ziziphi Spinosae can effectively improve the influence of rats' insomnia due to DL-4-chlorobenzene alanine,which is marked by increased number of independent activities of rats and recovery of some indexes such as 5-HT and 5-HIAA,etc.