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| 黄连提取物对肝细胞生长因子受体的特异性抑制作用 | |
| 引用本文： | 欧阳竞锋,李奇峰,林美娇.黄连提取物对肝细胞生长因子受体的特异性抑制作用[J].河南中医,2008,28(12):20-22. |
| 作者姓名： | 欧阳竞锋 李奇峰 林美娇 |
| 作者单位： | 1. 云南中医学院,云南,昆明,650200;浙江大学,浙江,杭州,310058 2. 云南中医学院,云南,昆明,650200 3. 昆明市儿童医院,云南,昆明,650034 |
| 基金项目： | 云南省教育厅资助项目 |
| 摘 要： | 目的:观察黄连提取物对HepG 2细胞生长的影响,探讨黄连提取物的作用机制。方法:HepG 2细胞培养基中加入肝细胞生长因子,形成过量表达受体酪氨酸激酶(RTK)的HepG 2细胞,加入黄连提取物,测定细胞生长活性、HGFR和P-HGFR的表达水平(活性)。结果:黄连提取物对HepG 2细胞生长、HGFR和P-HGFR的表达水平(活性)均有不同程度的抑制作用。结论:黄连提取物通过抑制RTK而抑制肿瘤细胞的生长。 |
| 关 键 词： | 黄连提取物 肝细胞生长因子受体 HepG2细胞 |
Experimental Study on Extract of Huanglian Inhibiting Hepatacyte Growth Factor Receptor Specifically |
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| Ouyang Jingfeng,Li Qifeng,Lin Meijiao.Experimental Study on Extract of Huanglian Inhibiting Hepatacyte Growth Factor Receptor Specifically[J].Henan Traditional Chinese Medicine,2008,28(12):20-22. | |
| Authors: | Ouyang Jingfeng Li Qifeng Lin Meijiao |
| Abstract: | Objective: To observe extract of Huanglian inhibiting the growth of HepG 2 and explore the mechanism.Methods: HepG 2 cells of excessive expressing receptor tyrosine kinases were established by adding hepatacyte growth factor(HGF) to MEM medium.Expressions of HGFR and P-HGFR were detected when supplemented extract of Huanglian or not.Results: Extract of Huanglian inhibited the growth of HepG 2,the expression of HGFR and the activity of HGFR to some extent.Conclusion: Extract of Huanglian inhibited the growth of HepG 2 by down-regulating expressions of HGFR and p-HGFR. |
| Keywords: | extract of Huanglian hepatacyte growth factor receptor HepG2 cells |
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