| HPLC法测定姜黄浸膏散中姜黄素含量 |
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| 引用本文： | 陶璐,陈科力,刘义梅,喻运珍,谢云.HPLC法测定姜黄浸膏散中姜黄素含量[J].亚太传统医药,2013,9(7):24-26. |
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| 作者姓名： | 陶璐 陈科力 刘义梅 喻运珍 谢云 |
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| 作者单位： | 陶璐 (湖北中医药大学,药学院,湖北,武汉,430065)； 陈科力 (湖北中医药大学,药学院,湖北,武汉,430065)； 刘义梅 (湖北中医药大学,药学院,湖北,武汉,430065)； 喻运珍 (武汉市水产科学研究所,湖北,武汉,430065)； 谢云 (湖北中医药大学,药学院,湖北,武汉,430065)； |
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| 基金项目： | 武汉市农科院2012年科技创新项目(项目编号：CX201218) |
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| 摘 要： | 目的：对姜黄浸膏散中的姜黄素进行含量测定。方法：采用高效液相色谱法,TIANHE C18（250mm×4.6mm,5μm）色谱柱,以乙腈-4%冰醋酸溶液（48∶52）为流动相,流速为1mL/min,检测波长为430nm,柱温为30℃。结果：HPLC法测定姜黄浸膏散中姜黄素的含量在4.383～21.92μg/mL范围内线性关系良好,r=0.999 8,平均加样回收率为98.67%,RSD=0.96%。结论：该法简单,准确,重现性好,可用于姜黄浸膏散的质量控制。
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| 关 键 词： | HPLC 姜黄浸膏散 姜黄素 药物含量测定 |
High Performance Liquid Chromatography Curcumin in Extract Powder of on Determination of Turmeric |
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| Tao Lu,Liu Yimei,Yu Yunzhen,Chen Keli,Xie Yun.High Performance Liquid Chromatography Curcumin in Extract Powder of on Determination of Turmeric[J].Asia-Pacific Traditional Medicine,2013,9(7):24-26. |
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| Authors: | Tao Lu Liu Yimei Yu Yunzhen Chen Keli Xie Yun |
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| Institution: | 1. College of Pharmacy, Hubei University of Chinese Medicine,Wuhan 430065, China; 2. College of Pharmacy, Hubei University of Chinese Medicine,Wuhan 430065,China; 3. Wuhan Aquatic Product Research Institute, Wuhan 430065, China) |
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| Abstract: | Objective=To establish a HPLC method for determination of eurcumin in extract powder of turmeric. Methods：The HPLC conditions with TIANHE-C18 column （250mm×4.6mm,5μm） was used, the mobile phase was a solution of acetonitrile-4 glacial acetic acid （48 = 52）;flow rate was 1.0mL/min, the detective wavelength was 430nm, column temperature was 30℃. Results： The determination of curcumin in extract powder of turmeric by HPLC was a good linear relationship at the range of 4. 383 to 21. 92μg, r=0. 999 8. The average recovery rate was 98.67% with RSD=0.96%. Conclusion： This method is simple, accurate and with good reproducibility, and can be used as the quality control standard for extract powder of turmeric. |
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| Keywords: | HPLC Extract Powder of Turmeric Curcumin Content Determination |
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