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两种滇产木姜子属植物中总黄酮的抗氧化活性研究
引用本文:王少杰,马水仙,张娜,周树娅,尹艳清,杨丽娟.两种滇产木姜子属植物中总黄酮的抗氧化活性研究[J].亚太传统医药,2013,9(1):19-21.
作者姓名:王少杰  马水仙  张娜  周树娅  尹艳清  杨丽娟
作者单位:1. 云南民族大学民族药资源化学国家民委-教育部重点实验室,云南昆明,650500
2. 云南民族大学民族药资源化学国家民委-教育部重点实验室,云南昆明650500;云南民族大学云南省聚乳酸基功能材料工程实验室,云南昆明650500
基金项目:国家自然科学基金项目(21162042);云南自然科学基金项目(2010CD090);云南省高校化学类核心课程教学团队项目
摘    要:目的:对滇产金平木姜子和红叶木姜子进行总黄酮含量的测定,并分别研究总黄酮的抗氧化活性。方法:用正丁醇分别浸提金平木姜子和红叶木姜子得到两种提取物,用紫外分光光度法测定各自总黄酮的含量,同时以DPPH清除率实验来评价其抗氧化活性并分别绘制DPPH随浓度变化曲线进行比较。结果:金平木姜子中黄酮提取物的DPPH自由基清除率最高为72.63%,IC50值为1.75mg/mL;红叶木姜子中黄酮提取物的DPPH自由基清除率最高为86.67%,IC50值为1.48mg/mL。结论:金平木姜子和红叶木姜子中含较丰富的总黄酮化合物,具有较强的抗氧化活性,且红叶木姜子中总黄酮的抗氧化活性高于金平木姜子。

关 键 词:金平木姜子  红叶木姜子  总黄酮  DPPH  抗氧化活性

Study on the Antioxidant Activities of Total Flavonoids from Two Plants of Litsea in Yunnan
Wang Shaojie , Ma Shuixian , Zhang Na , Zhou Shuya , Yin Yanqing , Yang Lijuan.Study on the Antioxidant Activities of Total Flavonoids from Two Plants of Litsea in Yunnan[J].Asia-Pacific Traditional Medicine,2013,9(1):19-21.
Authors:Wang Shaojie  Ma Shuixian  Zhang Na  Zhou Shuya  Yin Yanqing  Yang Lijuan
Institution:1,2(1.Key Laboratory of Ethnic Medicine Resource Chemistry,State Ethnic Affairs Commission & Ministry of Education,Yunnan University of Nationalities,Kunming 650500,China; 2.The Engineering Laboratory of Polylactic Acid-Based Functional Materials of Yunnan, Yunnan University of Nationalities,Kunming 650500,China)
Abstract:Objective:To determine the total flavonoids in Litsea chingpingensis and Litsea rubescens from Yunnan and investigate their antioxidant activities of DPPH. Methods: The flavonoids were extracted from the two plants of Litsea with n-butanol. Their contents were determined by UV-Vis spectrophotometry and the antioxidant activities were evaluated by the removal rate for DPPH. For further investigation, the standard curve could be drawn by using these data. Results:The DPPH removal rate of flavonoids in Litsea chingpingensis was 72.63 ; and its IC50 value was 1.75 mg/mL. While the DPPH removal rate of flavonoids in Litsea rubescens was 86. 67 ; and its IC50 value was 1.48mg/mL. Conclusion:The Litsea chingpingensis and Litsea rubescens were riched in flavonoids which had good antioxidant activities, and the antioxidant activity of Litsea rubescens is superior to that of Litsea chingpingensis.
Keywords:Litsea Chingpingensis  Litsea Rubescens  Total Flavonoids  DPPH  Antioxidant Activities
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