中药石韦中黄酮类成分微乳薄层色谱分析研究

目的：探究微乳薄层色谱及其图像分析在中药石韦中的鉴别应用。方法：以传统有机相和SDS-正丁醇-正庚烷-水微乳液作为展开剂，通过普通硅胶G板，高效硅胶薄膜和聚酰胺薄层层析，分离鉴别了中药石韦中黄酮类化学成分，考察了提取溶剂、微乳液类型、改性剂等因素对分离效果的影响。结果：中药石韦的最佳提取溶剂为60％乙醇，最佳展开溶剂为含水量75％的微乳溶液，加0．8mL甲酸和0．2mL丙酮作为改性剂。结论：该法分离效果显著，实验时间短，灵敏度高，各类组分可同时分析鉴定，点样量小，斑点清晰，圆而集中，且不使用刺激性强的有机溶剂，为石韦的鉴别提供了一种简便、准确方法。

Research on the Microemulsion TLC Identification Application in Pyrrosia Leaf

Objective：to explore the microemμLsion TLC identification application in pyrrosia leaf. Methods： SDS a n-butyl alcohol n-heptane monohydrate microemμLsion as expansion agent, through the polyamide thin layer chromatography, separation and i dentify the pyrrosia leaf flavonoids in medicinal chemistry, examines the extraction solvent, microemμLsion type, modifier, etc. Factors influencing the separation effect. Results： the pyrrosia leaf medicinal materials best extraction solvent was 60 % ethanol, the best solvent for moisture content 75 % microemμLsion solution,0.8 mL and 0.8 mL of formic acid and acetone as the modifier. Conclusion：the separation effect is remarkable, short test time, high sensitivity, various components can analysis appraisal at the same time, some small amount of sample,the spots were clear, round and concentrated,do not use excitant strong organic solvent, as the pyrrosia leaf identification provides a simple and accurate method.