大孔树脂分离纯化巴豆中木兰花碱工艺研究

目的:优化大孔树脂分离纯化巴豆中木兰花碱的工艺条件。方法:采用HPLC法测定木兰花碱,筛选AB-8,HPD400,ADS-17,NKA-9,D101树脂对木兰花碱的吸附和解吸附能力,并优化分离纯化条件。结果:优选出D101树脂,上样液浓度为生药1.5g/mL,吸附流速1.5BV/h,药材量:树脂量为6∶1;分离纯化条件为:先用4BV水冲洗除去不吸附杂质,再用3BV 8%乙醇除去大极性干扰杂质,最后用4BV 23%乙醇冲洗收集目标滤液。结论:该方法简便易行,适用于巴豆中木兰花碱的精制与纯化。

Separation and Purification of Magnoflorine from Croton Tiglium by Macroporous Resins

Objective:To study the optimal optimally purified process of magnoflorine extracted from Croton tiglium with macroporous resins.Methods:The HPLC was used to analyze the content of magnoflorine,the adsorption and desorption performances of AB-8,HPD400,ADS-17,NKA-9,D101 macroporous resins were compared and then optimize purification conditions.Results:D101 resin was selected because it gave the most effective of separation.The optimum process for the purification was as follows:the concentration of sample solution was 1.5 g/mL;adsorption flow rate was 1.5 BV/h;crude Medicinal materials weight than reisn weight for six.4 BV water 3 BV 8% ethanol and 4 BV 23% ethanol were used as eluant of Croton tiglium respectively.Portions of 23% ethanol were collected.Conclusion:This method is simple and feasible with good effect of enrichment in magnoflorine from Croton tiglium.