In Vitro activities of plant extracts from Saudi Arabia against malaria,leishmaniasis, sleeping sickness and Chagas disease |
| |
Authors: | Essam Abdel‐Sattar Louis Maes Maha Mahmoud Salama |
| |
Institution: | 1. Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia;2. Laboratory for Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, Antwerp University, Groenenborgerlaan 171, B‐2020 Antwerp, Belgium;3. Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt |
| |
Abstract: | The in vitro activity of the methanol extracts of 51 plants randomly collected from the Kingdom of Saudi Arabia and some of their fractions (petroleum ether, chloroform, ethyl acetate and aqueous) were evaluated against Plasmodium falciparum, Trypanosoma brucei brucei, T. cruzi and Leishmania infantum, as well as toxicity against MRC‐5 fibroblast cells. Ten crude methanolic extracts that demonstrated potent and adequately selective antiprotozoal activity were subjected to solvent fractionation using petroleum ether, ethyl acetate and chloroform. Only three samples showed promising antiprotozoal activity. Argemone ochroleuca (CHCl3 fraction) showed pronounced activity against P. falciparumGHA (IC50 0.32 μg/mL) and T. cruzi (IC50 0.30 μg/mL) with low cytotoxicity against MRC‐5 cells (CC50 11.6 μg/mL). Capparis spinosa (EtOAc fraction) showed pronounced activity against P. falciparumGHA with an IC50 0.50 μg/mL in the absence of toxicity against MRC‐5 cell line (CC50 > 30 μg/mL). Heliotropium curassavicum (CHCl3 fraction) showed similar activity against P. falciparum (IC50 0.65 μg/mL; MRC‐5 CC50 > 30 μg /mL). These three extracts will be subjected for further extensive studies to isolate and identify their active constituents. Copyright © 2010 John Wiley & Sons, Ltd. |
| |
Keywords: | in vitro antimalarial antitrypanosomal antileishmanial cytotoxicity |
|
|