姜黄素抗肺纤维化大鼠自由基损伤作用的实验研究

目的:研究姜黄素抗肺纤维化大鼠自由基损伤的作用和作用机制。方法:SD雄性大鼠,分为假手术组,模型组,醋酸泼尼松组,姜黄素高、中、低剂量组,每组24只。除假手术组外其余各组大鼠气管内一次性滴注盐酸博莱霉素,假手术组大鼠气管内一次性滴注等体积的生理盐水。造模后第1天开始给药,持续到处死动物的前一天。姜黄素高、中、低剂量组分别为200,100,50 mg.kg^-1.d^-1,醋酸泼尼松组分0.56 mg.kg^-1.d^-1,假手术组,模型组分别灌服等体积的生理盐水。分别测定7,14,28 d时大鼠肺组织谷胱甘肽过氧化物酶(GSH-Px),超氧化物歧化酶(SOD),丙二醛(MDA)和诱导型一氧化氮合酶(iNOS)的含量。结果:姜黄素能提高大鼠体内的GSH-Px和SOD水平,降低MDA水平和iNOS水平。结论:姜黄素能调节肺纤维化大鼠体内自由基水平,减轻自由基对肺组织结构的氧化损伤,是该药防治肺纤维化的作用机制。

Experimental study on effect of curcumin on inhibiting injury of free radical in pulmonary fibrosis rats

Objective:To study on the effect and mechanism of curcumin on inhibiting injury induced by free radical in pulmonary fi-brosis.Method:One hundred and forty-four male SD rats were randomly divided into 6 groups(24 rats in each group).Rats in the model con-trol group,positive medicine group,and high,moderate and low curcumin groups were injected with a single dose of bleomycin by trachea,and rats in sham-model control group with same volume normal saline.One day after the injection,curcumin solution of different dosages(200,100,50 mg.kg^-1.d^-1) was respectively given to rats in the high,moderate and low curcumin group by daily gastrogavage,while equal volume of normal saline was given to those in the sham-model control group and model control group,and an equal volume of prednisone(0.56 mg.kg^-1.d^-1) was saline was given to those in positive medicine control group.On the 7,14,28 days,the contents of GSH-Px,SOD,MDA and iNOS in pulmonary tissues of different groups were measured.Result:Curcumin can raise the content of SOD and GSH-Px and lessen the level of MDA and iNOS.Conclusion:Curcumin can regulate the level of free radical in the body of rats with pulmonary fibrosis and lessen the oxidative injury of pulmonary tissues caused by free radical,in the body of rats with pulmonary fibrosis.The mechanisms of curcumin on idiopathic pulmonary fibrosis lie in adjusting the level of free radical and inhibiting the injury of lung tissue induced by free radical.