地椒中1个新的二氢黄酮化合物

研究地椒的化学成分,利用硅胶,ODS,Sephadex LH-20和半制备HPLC等色谱方法分离纯化,结合其MS,NMR,UV,IR和CD等现代分析技术鉴定化合物的结构。从地椒的乙醇提取物乙酸乙酯萃取部位分离并鉴定了4个二氢黄酮化合物,分别为(2S)-5,6-dihydroxy-7,8,4'-trimethoxyflavanone(1),5,4'-dihydroxy-6,7-dimethoxyflavanone(2),(2S)-5,4'-dihydroxy-7,8-dimethoxyflavanone(3),樱花素(4)。其中,化合物1为新化合物,并通过CD谱确定其构型,化合物3为首次分离得到的天然产物,并通过CD谱确定其构型,化合物2为首次从百里香属分离得到,化合物4为首次从地椒中分离得到。体外细胞毒性试验结果表明,化合物1~4对人类肺癌细胞A549具有微弱的细胞毒性,IC₅₀为74.5~135.6μmol·L^-1。

A novel flavanone from Thymus przewalskii

This study was to investigate the chemical constituents from the aerial parts of Thymus przewalskii.The chemical constituents were separated and purified by column chromatography on silica gel,ODS,Sephadex LH-20 and semi-prepared HPLC,and their structures were determined by physicochemical properties and spectroscopic data.Four flavanones were isolated from the ethanol extract of the aerial parts of T.przewalskii,and identified as(2S)-5,6-dihydroxy-7,8,4'-trimethoxyflavanone(1),5,4'-dihydroxy-6,7-dimethoxyflavanone(2),(2S)-5,4'-dihydroxy-7,8-dimethoxyflavanone(3),sakuranetin(4),respectively.Compound 1 was a new compound and its configuration was determined by CD spectrum,compound 3 was natural product which was isolated for the first time and their configurations were determined by CD spectra.Compound 2 was isolated from the genus Thymus for the first time and compound 4 was isolated from T.przewalskii for the first time.Furthermore,cytotoxicity test was assayed for the four flavanones.They exhibited weak cytotoxicity against human lung cancer cells(A549),with the IC₅₀ from 74.5 to 135.6μmol·L^-1.