| 硫磺熏蒸对亳菊化学成分的影响 |
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| 引用本文： | 李友连,王珊,朱晶晶,王维皓,向世勰,冯伟红,陈两绵,王智民,陆兔林.硫磺熏蒸对亳菊化学成分的影响[J].中国中药杂志,2015,40(13):2624-2628. |
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| 作者姓名： | 李友连 王珊 朱晶晶 王维皓 向世勰 冯伟红 陈两绵 王智民 陆兔林 |
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| 作者单位： | 安徽中医药大学, 安徽 合肥 230000;中国中医科学院 中药研究所, 北京 100700,陕西国际商贸学院, 陕西 西安 712046,中国中医科学院 中药研究所, 北京 100700,中国中医科学院 中药研究所, 北京 100700,中国中医科学院 中药研究所, 北京 100700,中国中医科学院 中药研究所, 北京 100700,中国中医科学院 中药研究所, 北京 100700,中国中医科学院 中药研究所, 北京 100700,南京中医药大学 药学院, 江苏 南京 210023 |
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| 基金项目： | 国家中医药管理局行业专项(201307008-04);国家自然科学基金面上项目(81373960) |
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| 摘 要： | 该实验采用HPLC-ESI-MSn对硫磺熏蒸与晒干的亳菊进行定性比较,共指认了32个化合物。同时,采用HPLC技术对绿原酸、木犀草苷、异绿原酸A、木犀草素、芹菜素、香叶木素及金合欢素7个化合物进行定量比较,研究结果发现硫熏前后亳菊在化学组成上无较大差别,但是在含量上存在显著差异,硫熏后,黄酮苷类及奎宁酸类成分的含量降低,而黄酮苷元的含量升高;进一步对硫熏与晒干亳菊进行主成分分析(PCA)及偏小二乘聚类判别模式分析(PLS-DA),PCA分析结果显示,6批亳菊样品聚成2组,分别为晒干组和硫熏组;通过PLS-DA分析提取出对硫熏前后亳菊分组贡献率大于1(VIP> 1)的3个成分,分别为芹菜素、木犀草素和异绿原酸A。该实验结果表明硫熏对亳菊化学成分具有显著影响。
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| 关 键 词： | 硫磺熏蒸 亳菊 LC-MS PCA PLS-DA |
| 收稿时间： | 6/2/2015 12:00:00 AM |
Study on effects of sulfur fumigation on chemical constituents of Chrysanthemum morifolium cv.Boju |
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| LI You-lian,WANG Shan,ZHU Jing-jing,WANG Wei-hao,Xiang Shi-xie,FENG Wei-hong,CHEN Liang-mian,WANG Zhi-min and LU Tu-lin.Study on effects of sulfur fumigation on chemical constituents of Chrysanthemum morifolium cv.Boju[J].China Journal of Chinese Materia Medica,2015,40(13):2624-2628. |
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| Authors: | LI You-lian WANG Shan ZHU Jing-jing WANG Wei-hao Xiang Shi-xie FENG Wei-hong CHEN Liang-mian WANG Zhi-min and LU Tu-lin |
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| Institution: | Anhui University of Chinese Medicine, Hefei 230000, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Shaanxi Institute of International Trade & Commerce, Xi'an 712046, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China and College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China |
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| Abstract: | A comprehensively comparison of the chemical profiles between sun-drying BJ (NBJ) and sulfur-fumigated BJ (SBJ)was conducted by HPLC analysis and the discrepant peaks were identified or tentatively assigned by HPLC-ESI-MSn.A total of 32 chemical components were used for qualitative comparison.Meanwhile, a quantitative comparison of BJwere conducted by HPLC analysis and determining seven compounds from 3 NBJ and 3 SBJ samples dramatic chemical changes were found.After sulfur fumigation, the contents of flavonoids glycosides and phenolic acids were remarkably reduced, but the contents of flavonoids aglycones were significantly increased.Multivariate statistics, including principle component analysis (PCA) and partial least squares discriminate analysis (PLS-DA) were used to investigate the potential damaging effect of sulfr-fumigating process.The PCA score plots showed six samples were clearly classified into the sun-drying and sulfur-fumigating groups.And according to VIP> 1, the most important chemical markers were apigenin, luteolin and 3,5-dicaffeoylquninic acid which could be used to distinguish NBJ and SBJ samples.Combining the results of qualitative and quantitative analysis, it showed that the sulfur fumigation has a significant effect on BJ. |
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| Keywords: | sulfur fumigation Chrysanthemum morifolium cv Boju LC-MS PCA PLS-DA |
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