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大孔树脂精制苦荞总黄酮工艺
引用本文:于智峰,王敏.大孔树脂精制苦荞总黄酮工艺[J].中国中药杂志,2007,32(7):585-589.
作者姓名:于智峰  王敏
作者单位:西北农林科技大学,食品科学与工程学院,陕西,杨凌,712100
摘    要:目的:筛选分离苦荞总黄酮精制的最佳树脂型号及最佳工艺。方法:通过静态、动态吸附-解吸相结合的方法,以黄酮吸附率、解吸率为评价指标,综合评判确定最优工艺。结果:DM-2树脂分离效果较好,其最佳吸附工艺为:澄清的粗提物浸膏水溶液(黄酮质量分数20%),树脂柱径高比以1∶10,上样液pH 3~4,吸附流速3.0 mL·min-1 。最佳洗脱条件为:5 BV,pH 8,50%的乙醇溶液,洗脱流速3 mL·min-1,精制后黄酮质量分数超过60%。结论:采用DM-2树脂精制苦荞黄酮,操作简单,生产周期短,成本低廉,精制效果突出,有较高的工业生产应用价值。

关 键 词:苦荞黄酮  大孔树脂  吸附  解吸  工艺
文章编号:1001-5302(2007)07-0585-05
收稿时间:2006-05-12
修稿时间:2006-05-12

Technique of macroporous resin for tartary buckwheat total flavonoids
YU Zhi-feng; WANG Min.Technique of macroporous resin for tartary buckwheat total flavonoids[J].China Journal of Chinese Materia Medica,2007,32(7):585-589.
Authors:YU Zhi-feng; WANG Min
Institution:College of Food Science and Engineering, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China. hheather@etang.com
Abstract:OBJECTIVE: To investigatre appropriate macroporous resins and the optimal technological parameters of the purification process of tartary buckwheat total flavonoids. METHOD: Static and dynamic adsorption-desorption methods were adopted, and evaluated for separating efficiency by macroporous resins absorption rates, desorption rates. RESULT: The DM -2 macroporous resin had the best separating efficiency. The best absorbed condition is H2O as solvent of extracts, diameter vs height: 1: 10, pH 3 - 4, absorption power: 3.0 mL x min(-1). The optimum adsorption condition is the volume of 50% ethanol 80 mL (approximately 5 BV (resin bed volume) as eluting solvent, pH 8, the desorption power: 3.0 mL x min(-1). CONCLUSION: This technology is simple, quick and cost-effective, which is suitable for industrialization.
Keywords:tartary buckwheat  total flavonoids  macroporous resin  absorption  desorption  technique
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