低氧条件下四逆汤及组方药提取物对大鼠血管内皮细胞释放PGI2,TXA2和NO的影响

目的 :观察四逆汤提取物 (DRE)及其组方药提取物对低氧条件下大鼠血管内皮细胞释放血栓素A₂(TXA₂ )、前列环素 (PGI₂ )和一氧化氮 (NO)的影响。方法 :低氧处理的大鼠血管内皮细胞经四逆汤及其组方药提取物作用后 ,取条件培养液 ,采用放射免疫法及Griess反应测定培养液中的PGI₂ ,TXA₂ 和NO的代谢产物 6 酮 前列腺素F_1α(6-keto-PGF_1α) ,血栓素B₂ (TXB₂ )及亚硝酸盐 (NO₂ -)含量。结果 :与对照组比较 ,DRE、甘草提取物 (LE)、干姜提取物 (GE)、附子提取物 (AE)、甘草加附子提取物 (ALE)、干姜加附子提取物 (AGE)可以显著增加低氧处理的大鼠血管内皮细胞培养液中 6-keto-PGF_1α的含量及 6-keto-PGF_1α/TXB₂ 的比值 ,但对TXB₂ 的含量没有显著影响。DRE组对 6-keto-PGF_1α含量的影响显著高于LE ,GE ,AE ,ALE ,AGE组。DRE组对 6-keto-PGF_1α/TXB₂ 比值的影响显著高于GE ,AE ,ALE组。与对照组比较 ,DRE ,LE ,GE ,AE ,AGE ,ALE组显著提高血管内皮细胞培养液中NO₂^-的含量。DRE的药理作用显著高于GE ,AE ,ALE组。结论 :四逆汤提取物增加低氧处理的大鼠血管内皮细胞培养液中NO ,PGI₂含量及PGI₂ /TXA₂ 的比值。四逆汤提取物的药理作用强于组方药提取物。

Effects of the extracts from Decoction for resuscitation and its component herbs on PGI2, TXA2 and NO release from rat vascular endothelial cells under hypoxia in vitro

OBJECTIVE: To compare the effect of the extracts from Decoction for resuscitation (DRE) and its component herbs on prostacyclin (PGI2), thromboxane A2 (TXA2) and nitric oxide (NO) release from rat vascular endothelial cells under hypoxia. METHOD: After treatment with the extracts from DRE and its component herbs, the contents of 6-keto-prostaglandin F1alpha(6-keto-PGF1alpha), thromboxane B2 (TXB2) as well as nitrite (NO), which were degradation products of PGI2, TXA2 and NO respectively, in culture medium of rat vascular endothelial cells under hypoxia were measured with radioimmunoassay and Griess Reaction. RESULT: Compared with the control group, the results indicated that DRE, prepared licorice root extract (LE), dried ginger extract (GE), aconite root extract (AE), extracts of aconite root and prepared licorice root (ALE), extracts of aconite root and dried ginger (AGE) increased significantly the content of 6-keto-PGF1alpha and the ratio of 6-keto-PGF1alpha/TXB2, but had no effect on the content of TXB2 in culture medium of rat vascular endothelial cells under hypoxia. The content of 6-keto-PGF1alpha in the DRE group was higher than that in the groups of LE, GE, AE, ALE, AGE. The ratio of 6-keto-PGF1alpha/TXB2 in the DRE group was higher than that of the groups of GE, AE, ALE. Compared with the control group, DRE, LE, GE, AE, ALE, AGE increased significantly the content of NO2- in culture medium of rat vascular endothelial cells under hypoxia. Moreover, the content of NO2- in the DRE group was higher than that of the groups of GE, AE, ALE. CONCLUSION: The results suggested that DRE increased significantly the content of PGI2 and the ratio of PGI2/TXA2 as well as the content of NO. The effect of DRE on the parameters in culture medium of rat vascular endothelial cells under hypoxia was better than that of the extracts from its component herbs.